Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis

Science Advances
Arata HondaChihiro Koshimoto

Abstract

In mammals, the Y chromosome strictly influences the maintenance of male germ cells. Almost all mammalian species require genetic contributors to generate testes. An endangered species, Tokudaia osimensis, has a unique sex chromosome composition XO/XO, and genetic differences between males and females have not been confirmed. Although a distinctive sex-determining mechanism may exist in T. osimensis, it has been difficult to examine thoroughly in this rare animal species. To elucidate the discriminative sex-determining mechanism in T. osimensis and to find a strategy to prevent its possible extinction, we have established induced pluripotent stem cells (iPSCs) and derived interspecific chimeras using mice as the hosts and recipients. Generated iPSCs are considered to be in the so-called "true naïve" state, and T. osimensis iPSCs may contribute as interspecific chimeras to several different tissues and cells in live animals. Surprisingly, female T. osimensis iPSCs not only contributed to the female germ line in the interspecific mouse ovary but also differentiated into spermatocytes and spermatids that survived in the adult interspecific mouse testes. Thus, T. osimensis cells have high sexual plasticity through which female soma...Continue Reading

References

Mar 13, 1986·Nature·P S BurgoyneA McLaren
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·S K BronsonJ T Mascarello
Nov 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A McLaren
Mar 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ayako IsotaniMasaru Okabe
Apr 15, 2005·Genesis : the Journal of Genetics and Development·Rie ShimizukawaKen-ichi Yagami
Feb 13, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Tsuyoshi KobayashiAsato Kuroiwa
May 24, 2008·Nature·Qi-Long YingAustin Smith
Jun 6, 2009·Cell Stem Cell·Jennifer Nichols, Austin Smith
May 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jacob HannaRudolf Jaenisch
May 6, 2010·Chromosoma·Asato KuroiwaYoichi Matsuda
Dec 7, 2010·The Journal of Biological Chemistry·Masahito IkawaMasaru Okabe
Mar 16, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ayako IsotaniMasaru Okabe
Sep 6, 2011·Nature Methods·Inbar Friedrich Ben-NunJeanne F Loring
Feb 22, 2013·Biology of Reproduction·Yoshinori KanemoriTadashi Baba
Mar 22, 2013·BioResearch Open Access·Rajneesh VermaPaul John Verma
May 7, 2015·Nature·Jun WuJuan Carlos Izpisua Belmonte
Oct 18, 2015·BMC Research Notes·Krishna RamaswamyJoseph G Hacia
Nov 7, 2015·Cell Stem Cell·Jun Wu, Juan Carlos Izpisua Belmonte
Mar 25, 2016·Biology of Reproduction·Toshiya Nishimura, Minoru Tanaka
Jul 19, 2016·Cell Stem Cell·Thorold W TheunissenRudolf Jaenisch

❮ Previous
Next ❯

Citations

Apr 11, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Arata Honda
Aug 12, 2020·Cellular Reprogramming·Érika Almeida PraxedesAlexsandra Fernandes Pereira
Feb 16, 2018·Annual Review of Animal Biosciences·Oliver A Ryder, Manabu Onuma
May 1, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Teruko Taketo
Oct 8, 2020·International Journal of Molecular Sciences·Lucie Gavin-PlagneMarielle Afanassieff
May 24, 2020·Genomics, Proteomics & Bioinformatics·Shahzina KanwalXichen Bao

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
transgenic
Fluorescence
PCR
cesarean section
Laser
Scanning microscopy

Software Mentioned

BZII

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biology of Reproduction
Jian Min DengRichard R Behringer
Current Opinion in Genetics & Development
Shoudong YeQi-Long Ying
© 2022 Meta ULC. All rights reserved