FLEXIBLE CULM 1 encoding a cinnamyl-alcohol dehydrogenase controls culm mechanical strength in rice.

Plant Molecular Biology
Xiangjun LiChangyin Wu

Abstract

Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including late heading time, semi-dwarf habit, and flexible culm. In this study, we cloned the FLEXIBLE CULM1 (FC1) gene in rice using a T-DNA tagging approach. FC1 encodes a cinnamyl-alcohol dehydrogenase and is mainly expressed in the sclerenchyma cells of the secondary cell wall and vascular bundle region. In these types of cells, a deficiency of FC1 in the fc1 mutant caused a reduction in cell wall thickness, as well as a decrease in lignin. Extracts from the first internodes and panicles of the fc1 plants exhibited drastically reduced cinnamyl-alcohol dehydrogenase activity. Further histological and biochemical analyses revealed that the p-hydroxyphenyl and guaiacyl monomers in fc1 cell wall were reduced greatly. Our results indicated that FC1 plays an important role in the biosynthesis of lignin and the control of culm strength in rice.

References

Dec 1, 1969·Analytical Biochemistry·D M Updegraff
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Sep 1, 1995·The Plant Journal : for Cell and Molecular Biology·Y G LiuR F Whittier
Jul 11, 1997·Science·J RalphR R Sederoff
Jul 24, 1998·The Plant Journal : for Cell and Molecular Biology·C HalpinG A Foxon
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Jun 8, 2001·The Plant Journal : for Cell and Molecular Biology·L JonesS R Turner
Jun 26, 2001·Phytochemistry·L A Donaldson
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Changyin WuQifa Zhang
Sep 25, 2003·Annual Review of Plant Biology·Wout BoerjanMarie Baucher
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Nicholas C. Carpita
Aug 16, 2005·Nature·UNKNOWN International Rice Genome Sequencing Project

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Citations

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Aug 24, 2012·Applied Biochemistry and Biotechnology·Aaron J SaathoffGautam Sarath
Sep 14, 2011·Plant Cell Reports·Ko HiranoMakoto Matsuoka
Jan 26, 2011·Plant Molecular Biology·Xiaoxia DaiChangyin Wu
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Aug 25, 2015·Journal of Integrative Plant Biology·Jinmi YoonGynheung An
Aug 25, 2015·Plant, Cell & Environment·Shuen-Fang LoSu-May Yu
Dec 20, 2014·Transgenic Research·Sanjana NegiT R Ganapathi
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Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Yumiko Arai-SanohMotohiko Kondo
Oct 7, 2015·Journal of Genetics·Jieqin LiYanlong Liu
Jun 3, 2017·Journal of Integrative Plant Biology·Peng HuangMiyako Ueguchi-Tanaka
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