DOI: 10.1101/481580Nov 28, 2018Paper

Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy

BioRxiv : the Preprint Server for Biology
Michal SlezakMatthew G Holt

Abstract

In utero electroporation is increasingly used for manipulation of gene expression in the mammalian central nervous system. Here we created a transposon-based single plasmid system, that allows long-term conditional loss- and gain-of-function experiments across major CNS cell types. The system is widely applicable across species, allowing fast, flexible and cheap cell manipulation in previously non-transgenic species.

Related Concepts

Gene Expression
Plasmids
Vertebrates
Plants, Transgenic
Genetic Manipulation
Research Study
Species

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