Flexible open-source automation for robotic bioengineering

BioRxiv : the Preprint Server for Biology
E. J. ChoryKevin M Esvelt


Liquid handling robots have become a biotechnology staple, allowing laborious or repetitive protocols to be executed in high-throughput. However, software narrowly designed to automate traditional hand-pipetting protocols often struggles to harness the full capabilities of robotic manipulation. Here we present Pyhamilton, an open-source Python package that eliminates these constraints, enabling experiments that could never be done by hand. We used Pyhamilton to double the speed of automated bacterial assays over current software and execute complex pipetting patterns to simulate population dynamics. Next, we incorporated feedback-control to maintain hundreds of remotely monitored bacterial cultures in log-phase growth without user intervention. Finally, we applied these capabilities to comprehensively optimize bioreactor protein production by maintaining and monitoring fluorescent protein expression of nearly 500 different continuous cultures to explore the carbon, nitrogen, and phosphorus fitness landscape. Our results demonstrate Pyhamilton's empowerment of existing hardware to new applications ranging from biomanufacturing to fundamental biology.

Related Concepts

Body Fluids
White Matter

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Cell Aging (Preprints)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Related Papers

Journal of the American College of Radiology : JACR
Luciano Prevedello, Ramin Khorasani
The Virtual Mentor : VM
Adrian Gropper
The Journal of Physical Chemistry Letters
Anna I KrylovHans-Joachim Werner
© 2020 Meta ULC. All rights reserved