Flexor tendon tissue engineering: bioreactor cyclic strain increases construct strength

Tissue Engineering. Part a
Sepideh SaberJames Chang

Abstract

Mutilating injuries of the hand and upper extremity result in tendon losses too great to be replaced by autologous grafts. The goal of this study was to use tissue engineering techniques to produce additional tendon material. We used a custom bioreactor to apply cyclic mechanical loading onto tissue-engineered tendon constructs to study ultimate tensile stress (UTS) and elastic modulus (E). Constructs used were acellularized rabbit hindpaw flexor digitorum profundus equivalents reseeded with tenocytes or left unseeded. Tendon constructs were subjected to a stretch force of 1.25 N over a 5-day course. Seeded tendon constructs that were exposed to bioreactor loading had a significantly increased UTS (71.17 +/- 14.15 N) compared to nonloaded controls (35.69 +/- 5.62 N) (p = 0.001). Similarly, seeded constructs exposed to bioreactor loading also had a significantly higher E (1091 +/- 169 MPa) compared to nonloaded controls (632 +/- 86 MPa) (p = 0.001). This study shows that cyclic loading of tendon constructs increases the UTS and elastic modulus of seeded constructs. The use of the bioreactor may therefore accelerate the in vitro production of strong, nonimmunogenic tendon material that can potentially be used clinically to recons...Continue Reading

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Citations

May 27, 2011·Tissue Engineering. Part a·Colin Y L WoonJames Chang
Oct 18, 2012·Tissue Engineering. Part B, Reviews·Tao WangMing H Zheng
Mar 19, 2011·Tissue Engineering. Part C, Methods·Jeffrey A PatenJeffrey W Ruberti
Jun 29, 2013·Plastic and Reconstructive Surgery·Joel A BronsteinJames Chang
Jun 29, 2013·Drug Discovery Today·Daniela CigogniniDimitrios Zeugolis
Feb 18, 2016·Stem Cells International·Arianna B LovatiMatteo Moretti
Sep 2, 2011·Biomaterials·Steven R CaliariBrendan A C Harley
Feb 4, 2016·Stem Cells International·Daniel W Youngstrom, Jennifer G Barrett
Dec 18, 2012·Biotechnology and Bioengineering·Tao WangMing H Zheng
Jun 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·Juan A MonteroJuan M Hurlé
May 28, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kojun TorigoeToshiko Yoshida
Aug 7, 2012·Journal of Tissue Engineering and Regenerative Medicine·Marco GovoniEmanuele Giordano
Nov 2, 2016·Connective Tissue Research·Daniel W YoungstromJennifer G Barrett
Mar 2, 2016·Biotechnology Progress·Ying Lei, Zannatul Ferdous
Sep 17, 2011·The Journal of Hand Surgery, European Volume·J ThorfinnJ Chang
Nov 23, 2013·The Journal of Hand Surgery, European Volume·M G GalvezJ Chang
May 22, 2018·The International Journal of Artificial Organs·Manuela Teresa RaimondiGiuseppe M Peretti
May 16, 2018·Journal of Tissue Engineering and Regenerative Medicine·Qian LiuChunfeng Zhao
Dec 24, 2010·Regenerative Medicine·Limin WangMichael S Detamore
Oct 4, 2018·The American Journal of Sports Medicine·Darryl D D'LimaSeong Hwan Moon
Jan 27, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Diana GasparDimitrios I Zeugolis
Dec 24, 2015·Biofabrication·Jessica SnyderWei Sun
Sep 18, 2020·Expert Review of Medical Devices·María Carmen Araque-MonrósJorge Más-Estellés
Sep 15, 2020·Advanced Biosystems·N CastroS Lanceros-Mendez
Jan 18, 2019·Tissue Engineering. Part a·Mitchell R LaddDavid J Hackam
Apr 26, 2020·International Journal of Molecular Sciences·Mersedeh TohidnezhadThomas Pufe
Apr 3, 2020·Biomedical Materials·Devina JaiswalSangamesh G Kumbar
Dec 22, 2017·Annals of the New York Academy of Sciences·Kaitlyn E WhitneyJohnny Huard
Apr 10, 2019·Annals of Biomedical Engineering·Giuseppe TalòArianna B Lovati
Sep 18, 2020·International Journal of Molecular Sciences·Maria Rita CiteroniBarbara Barboni

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