Fli1 acts at the top of the transcriptional network driving blood and endothelial development.

Current Biology : CB
Feng LiuRoger Patient

Abstract

Blood and endothelium arise in close association during development, possibly from a common precursor, the hemangioblast [1-4]. Genes essential for blood and endothelial development contain functional ETS binding sites, and binding and expression data implicate the transcription factor, friend leukaemia integration 1 (Fli1) [5-10]. However, loss-of-function phenotypes in mice, although suffering both blood and endothelial defects, have thus far precluded the conclusion that Fli1 is essential for these two lineages [11, 12]. By using Xenopus and zebrafish embryos, we show that loss of Fli1 function results in a substantial reduction or absence of hemangioblasts, revealing an absolute requirement. TUNEL assays show that the cells are eventually lost by apoptosis, but only after the regulatory circuit has been disrupted by loss of Fli1. In addition, a constitutively active form of Fli1 is sufficient to induce expression of key hemangioblast genes, such as Scl/Tal1, Lmo2, Gata2, Etsrp, and Flk1. Epistasis assays show that Fli1 expression is induced by Bmp signaling or Cloche, depending on the hemangioblast population, and in both cases Fli1 acts upstream of Gata2, Scl, Lmo2, and Etsrp. Taken together, these results place Fli1 at th...Continue Reading

References

Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·H W DetrichL I Zon
Oct 13, 2000·Cell·A Ciau-UitzR Patient
Feb 15, 2001·Oncogene·A H Truong, Y Ben-David
Apr 20, 2002·The Journal of Biological Chemistry·Henner Friedle, Walter Knöchel
May 25, 2002·Developmental Biology·Eric H DavidsonHamid Bolouri
Feb 1, 2003·Gene·Tsuneyuki Oikawa, Toshiyuki Yamada
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Carl-Johan ZettervallDan Hultmark
Dec 25, 2004·Developmental Biology·Kimberly A DooleyLeonard I Zon
Dec 13, 2005·PLoS Biology·Saulius Sumanas, Shuo Lin
Aug 1, 2006·Developmental Biology·Masafumi InuiMakoto Asashima
Oct 3, 2006·Developmental Cell·Hiroo Ueno, Irving L Weissman
Nov 28, 2006·Developmental Biology·Van N PhamBrant M Weinstein
Oct 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·John E PimandaBerthold Göttgens

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Citations

Oct 27, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christophe LancrinGeorges Lacaud
Aug 23, 2011·Frontiers of Medicine·Panpan Zhang, Feng Liu
Oct 24, 2013·Current Rheumatology Reports·Yoshihide Asano, Shinichi Sato
Jun 17, 2009·Journal de la Société de biologie·Alexandra Le Bras, Fabrice Soncin
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Toshiyuki TsunodaSenji Shirasawa
Mar 13, 2012·Current Opinion in Hematology·Kelly Lammerts van Bueren, Brian L Black
Mar 20, 2009·Development·Tessa PeterkinRoger Patient
May 28, 2013·Cellular and Molecular Life Sciences : CMLS·Igor M Samokhvalov
Aug 10, 2013·Blood Cells, Molecules & Diseases·Aldo Ciau-UitzFeng Liu
Nov 16, 2011·Current Rheumatology Reports·Bradley J Rabquer, Alisa E Koch
Jul 2, 2010·Human Cell·Akira KurisakiMakoto Asashima
Jul 30, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Michael P SarrasRobert V Intine
Oct 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Miki TakeuchiMakoto Kobayashi
Dec 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rimpei MoritaAkihiko Yoshimura
Aug 12, 2014·Science China. Life Sciences·YiJiang ZhouLi Zhang
Jul 29, 2010·Journal of Dermatological Science·Yoshihide AsanoMaria Trojanowska
Sep 15, 2015·Developmental Biology·Mizuho S MimotoJan L Christian
May 15, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Makoto AsashimaTakashi Ariizumi
Feb 24, 2010·The Journal of Dermatology·Yoshihide Asano
Mar 17, 2010·The American Journal of Pathology·Yoshihide AsanoMaria Trojanowska
Feb 24, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mounia BoulberdaaAndrew H Baker
Jun 19, 2012·Biochimica Et Biophysica Acta·Chunxia ZhangFeng Liu
Oct 17, 2012·Biochimica Et Biophysica Acta·P KaimakisE Dzierzak
Oct 4, 2012·Mechanisms of Development·Sheran H W Law, Thomas D Sargent
Mar 1, 2011·Developmental Biology·Caramai N KameiThomas M Schultheiss

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