Flightin is necessary for length determination, structural integrity, and large bending stiffness of insect flight muscle thick filaments

Journal of Molecular Biology
John L ContompasisJim O Vigoreaux

Abstract

Despite the fundamental role of thick filaments in muscle contraction, little is known about the mechanical behavior of these filaments and how myosin-associated proteins dictate differences between muscle types. In this study, we used atomic force microscopy to study the morphological and mechanical properties of fully hydrated native thick filaments isolated from indirect flight muscle (IFM) of normal and mutant Drosophila lacking flightin (fln(0)). IFM thick filaments from newly eclosed (0-1 h old) wild-type flies have a mean length of 3.04+/-0.05 microm. In contrast, IFM thick filaments from newly eclosed fln(0) flies are more variable in length and, on average, are significantly longer (3.90+/-1.33 microm) than wild-type filaments from flies of the same age. In the absence of flightin, thick filaments can attain lengths >300% of wild-type filaments, indicating that flightin is required for setting the proper filament length in vivo. Filaments lacking flightin are structurally compromised, and filament preparations from fully matured 3- to 5-day-old adult fln(0) IFM yielded fragments of variable length much shorter than 3.20+/-0.04 microm, the length obtained from wild-type flies of similar age. The persistence length, an i...Continue Reading

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Citations

Jul 14, 2010·Journal of Biomedicine & Biotechnology·Mark S MillerJim O Vigoreaux
Apr 15, 2015·Journal of Molecular Biology·Zacharias OrfanosJohn Sparrow
Nov 26, 2013·Journal of Molecular Evolution·Felipe N Soto-AdamesJim O Vigoreaux
Mar 1, 2012·Journal of Molecular Biology·Sheetal S SalviUpendra Nongthomba
Nov 13, 2019·Scientific Reports·Francois Olivier HebertRoger C Levesque
Jul 8, 2020·Cells·Preethi Poovathumkadavil, Krzysztof Jagla
Nov 28, 2012·Journal of Cell Science·Zacharias Orfanos, John C Sparrow

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