FLJ25439, a novel cytokinesis-associated protein, induces tetraploidization and maintains chromosomal stability via enhancing expression of endoplasmic reticulum stress chaperones

Cell Cycle
Tai-Long PanPin Ouyang

Abstract

Investigation of the mechanisms leading to aneuploidy and polyploidy is critical to cancer research. Previous studies have provided strong evidence of the importance of tetraploidization as an early step in tumorigenesis. In cancer cells, tetraploid cells may contribute to abnormal mitotic progression, which may be associated with cytokinesis failure. Tetraploidy leads to genomic instability due to centrosome and chromosome over-replication. Until now, the mechanism by which cells maintain tetraploid status has been unknown. Here, we identified a novel D box-containing protein, FLJ25439, which displays a dynamic expression profile during mitosis/cytokinesis with the midbody as the most prominent associated structure. To understand the function of FLJ25439, we established stable cell lines overexpressing FLJ25439. FLJ25439-overexpression cells grew slower and displayed a tetraploid DNA content in comparison with diploid parental cells. They also showed aberrant mitosis and dysregulated expression of p53, pRb and p21, suggesting a defect in cell cycle progression. To explore the molecular mechanisms responsible for FLJ25439-induced tetraploidization, we conducted a comparative analysis of the global protein expression patterns of...Continue Reading

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Datasets Mentioned

BETA
JN799997
FLJ25439

Methods Mentioned

BETA
electrophoresis
GTPase
2 hybrid
transfection
flow cytomerty
flow cytometry
protein folding
PCR
Protein Assay

Software Mentioned

Ensembl
BioTools
Mascot
Nonlinear Progenesis
GeneTools
MetaCore
GeneGoMetaCore
Clustalw
Prodigy SameSpots
SPSS

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