PMID: 8955395Dec 1, 1996Paper

FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin

Journal of Bacteriology
W S Lo, A M Dranginis

Abstract

We report the characterization of a gene encoding a novel flocculin related to the STA genes of yeast, which encode secreted glucoamylase. The STA genes comprise sequences that are homologous to the sporulation-specific glucoamylase SGA and to two other sequences, S2 and S1. We find that S2 and S1 are part of a single gene which we have named FLO11. The sequence of FLO11 reveals a 4,104-bp open reading frame on chromosome IX whose predicted product is similar in overall structure to the class of yeast serine/threonine-rich GPI-anchored cell wall proteins. An amino-terminal domain containing a signal sequence and a carboxy-terminal domain with homology to GPI (glycosyl-phosphatidyl-inositol) anchor-containing proteins are separated by a central domain containing a highly repeated threonine- and serine-rich sequence. Yeast cells that express FLO11 aggregate in the calcium-dependent process of flocculation. Flocculation is abolished when FLO11 is disrupted. The product of STA1 also is shown to have flocculating activity. When a green fluorescent protein fusion of FLO11 was expressed from the FLO11 promoter on a single-copy plasmid, fluorescence was observed in vivo at the periphery of cells. We propose that FLO11 encodes a floccul...Continue Reading

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Citations

Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·M A VivierI S Pretorius
Jul 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Manuel FidalgoJuan Jimenez
May 8, 2009·Bioscience, Biotechnology, and Biochemistry·Sanom NonklangRinji Akada
Aug 28, 2009·Critical Reviews in Biotechnology·Pardeep Kumar, T Satyanarayana
Jan 29, 2010·PLoS Genetics·Joshua A Granek, Paul M Magwene
Nov 13, 2010·PLoS Biology·Chia-Yung WuGerald R Fink
Feb 25, 2011·Astrobiology·Sebastiaan E Van MuldersRonnie Willaert
Jun 18, 2011·Microbiology·Christoph BuerthDenis Tielker
Aug 11, 2016·Applied Microbiology and Biotechnology·Martina AvbeljPeter Raspor
Jan 16, 2018·Applied Microbiology and Biotechnology·Ke ZhangDao-Qiong Zheng
May 3, 2018·FEMS Yeast Research·Libuše Váchová, Zdena Palková
Jun 26, 2018·PLoS Genetics·Kaitlyn L NormanAnuj Kumar
Mar 8, 2019·MSphere·Jacky ChowPaul J Cullen
May 12, 2001·FEMS Microbiology Reviews·C A D'Souza, J Heitman
Oct 22, 2005·Yeast·Michael J Svarovsky, Sean P Palecek
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Mar 25, 2010·PLoS Genetics·Colin A ChavelPaul J Cullen
Aug 3, 2010·Applied Microbiology and Biotechnology·Florian F BauerMichael C Bester
Feb 16, 2012·FEMS Immunology and Medical Microbiology·Rasmus K BojsenBirgitte Regenberg
Jul 24, 2012·FEMS Yeast Research·Mahbuba H Meem, Paul J Cullen
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Oct 21, 2015·PLoS Genetics·Matthew B Taylor, Ian M Ehrenreich
Sep 5, 2019·Proceedings. Biological Sciences·R M Fisher, B Regenberg

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