Floating lipid bilayers deposited on chemically grafted phosphatidylcholine surfaces

Langmuir : the ACS Journal of Surfaces and Colloids
Arwel V HughesStephen J Roser

Abstract

Floating supported bilayers (FSBs) are new systems which have emerged over the past few years to produce supported membrane mimics, where the bilayers remain associated with the substrate, but are cushioned from the substrates constraining influence by a large hydration layer. In this paper we describe a new approach to fabricating FSBs using a chemically grafted phospholipid layer as the support for the floating membrane. The grafted lipid layer was produced using a Langmuir-Schaeffer transfer of acryloyl-functionalized lipid onto a pre-prepared substrate, with AIBN-induced cross-polymerization to permanently bind the lipids in place. A bilayer of DSPC was then deposited onto this grafted monolayer using a combination of Langmuir-Blodgett and Langmuir-Schaeffer transfer. The resulting system was characterized by neutron reflection under two water contrasts, and we show that the new system shows a hydrating layer of approximately 17.5 A in the gel phase, which is comparable to previously described FSB systems. We provide evidence that the grafted substrate is reusable after cleaning and suggest that this greatly simplifies the fabrication and characterization of FSBs compared to previous methods.

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Citations

Oct 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Kirk MulliganLinda J Johnston
May 13, 2009·Molecular Pharmaceutics·Chiranjeevi PeetlaVinod Labhasetwar
Aug 7, 2009·Journal of the Royal Society, Interface·Jeremy H Lakey
Oct 18, 2013·Journal of the Royal Society, Interface·Luke A CliftonStephen A Holt
Nov 26, 2013·Advances in Colloid and Interface Science·Thomas Günther PomorskiMarité Cárdenas
Jul 18, 2014·Journal of the Royal Society, Interface·Arwel V HughesJeremy H Lakey
Mar 29, 2014·Biochimica Et Biophysica Acta·Frank Heinrich, Mathias Lösche
Dec 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Luke A CliftonJeremy H Lakey
Aug 5, 2015·Chemistry and Physics of Lipids·F A HeberleJ Katsaras
Jun 28, 2016·Angewandte Chemie·Luke A CliftonJeremy H Lakey
Sep 4, 2015·Angewandte Chemie·Luke A CliftonJeremy H Lakey
Jan 17, 2012·Journal of Colloid and Interface Science·Timothy A OlesonAnthony J Giuffre
Oct 26, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Yuri GerelliGiovanna Fragneto
Apr 16, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Frédéric F Harb, Bernard Tinland
Dec 6, 2016·Acta Crystallographica. Section D, Structural Biology·Arwel V HughesJohn R P Webster
Jan 4, 2019·Acta Crystallographica. Section D, Structural Biology·Olga B FlorekRichard A Frazier
Oct 3, 2020·Biochemical Society Transactions·Nicolò ParaciniJeremy H Lakey
Feb 12, 2020·Advances in Colloid and Interface Science·Luke A CliftonMarité Cárdenas
Nov 25, 2018·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Reg RehalRichard D Harvey
May 12, 2010·The Journal of Physical Chemistry. B·Marie-Louise AinalemTommy Nylander
Aug 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Jonathan P TalbotGiovanna Fragneto
Dec 19, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Anne CharrierGregory P Lopinski
Apr 12, 2021·Journal of Colloid and Interface Science·Laura H JohnLuke A Clifton
Sep 26, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Luke Ashley CliftonMaximilian W A Skoda

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