Flow-based systems for rapid and high-precision enzyme kinetics studies.

Journal of Analytical Methods in Chemistry
Supaporn Kradtap Hartwell, Kate Grudpan

Abstract

Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics studies can be carried out at real-time initial rate avoiding the potential errors inherent in manual operation. Flow-based systems have been developed to provide rapid, low-volume, and high-precision analyses that effectively replace the many tedious and high volume requirements of conventional wet chemistry analyses. This article presents various arrangements of flow-based techniques and their potential use in future enzyme kinetics applications.

References

Dec 10, 2002·Science·Mohammad Movassaghi, Eric N Jacobsen
Oct 24, 2003·Analytical Chemistry·Xianzhi ZhouMichael D Toney
Oct 29, 2003·Journal of Biotechnology·Viera IlleováMohammad Juma
Jul 13, 2004·Trends in Biotechnology·Jean-Philippe Goddard, Jean-Louis Reymond
Dec 31, 2005·Journal of Molecular Recognition : JMR·Juankun Zhang, Anthony E G Cass
Jan 13, 2006·Biomedical Microdevices·Masaki YamaguchiJunichi Wakasugi
Jan 25, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yan ChenJaromir Ruzicka
Feb 8, 2006·Bioelectrochemistry·Fu-Shiang ChangTzong-Jih Cheng
Dec 26, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kerstin Schöringhumer, Margit Cichna-Markl
Feb 15, 2007·Analytical Chemistry·Dongeun HuhShuichi Takayama
Mar 27, 2007·Biosensors & Bioelectronics·Norihiro NashidaHiroaki Suzuki
Sep 29, 2007·Journal of Pharmaceutical and Biomedical Analysis·Katerina MervartováJosé Martínez Calatayud
Oct 2, 2007·Analytica Chimica Acta·Dominika Ogończyk, Robert Koncki
Dec 22, 2007·Annals of the New York Academy of Sciences·Jean-Louis Reymond
May 27, 2008·Analytica Chimica Acta·Mário A FeresJosé L F C Lima
Oct 31, 2008·Talanta·K K Stewart, S W Hillard
May 19, 2009·Journal of Molecular Biology·Gemma L HollidayJanet M Thornton
Jan 13, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Supaporn Kradtap HartwellKate Grudpan
Jul 29, 2010·Electrophoresis·Jaroslava SvobodováAles Svatos
Dec 1, 2010·Analytical and Bioanalytical Chemistry·Regina BleulKlaus S Drese
Mar 15, 2011·Analytica Chimica Acta·Cristina I C SilvestreJosé L F C Lima
May 24, 2011·Journal of Automated Methods & Management in Chemistry·Wang Lai-Hao, Huang Shu-Juan

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Citations

Apr 19, 2017·Analytical Chemistry·Tomer NoyhouzerJanine Mauzeroll
Oct 2, 2017·The Journal of Chemical Physics·Xiangying Guan, Raj Chakrabarti

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Methods Mentioned

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