Flow cytometric analyses of the subclasses of red cell IgG antibodies

Vox Sanguinis
R LynenM Köhler

Abstract

We report on flow cytometric IgG subclass determinations of red cell antibodies using polyclonal FITC-labeled antibodies. The limit of detection of this method was 1 ng anti-D per 1 x 10(7) red cells. The inter- and intra-assay coefficients of variance were 8.2 and 2.3%, respectively. In 8 newborns with a positive direct antiglobulin test (DAT) in the gel centrifugation test (GCT), due to ABO antibodies, IgG1 was detected in all and IgG2 additionally in 4 of these cases. In 5 severe cases of hemolytic disease of the newborn (HDN) due to anti-D, large amounts of IgG1 were found, and in 3 of these 5, IgG3 in combination with IgG1. In 8 mild or moderate HDN cases (4 anti-D, 2 anti-E, 1 anti-Fya, 1 anti-Jka), phototherapy sufficed, and IgG1 was the only antibody. In 7 adult patients with malignant lymphoma and a positive DAT (GCT), only small amounts of IgG1 red cell autoantibodies could be demonstrated by flow cytometry. In 5 further patients with malignant lymphoma, a positive DAT, and severe hemolytic anemia, large amounts of IgG1 autoantibodies were found and IgG3 was also present in 3 of these cases. Flow-cytometric determination of IgG subclasses may be a useful tool in immunohematology, since subclass determinations were pos...Continue Reading

References

Jun 1, 1986·American Journal of Obstetrics and Gynecology·M M TaslimiJ V Dacus
May 1, 1993·British Journal of Haematology·B ZupanskaR Stamps

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Citations

Jan 25, 2000·Journal of Clinical Laboratory Analysis·S J Alié-DaramJ M Dugoujon
Jan 1, 1997·Vox Sanguinis·J Fabijańska-MitekB Zupańska
May 29, 2000·Transfusion·T WagnerG Lanzer
Jun 7, 2011·Seminars in Perinatology·Sharon Markham Geaghan
Sep 15, 2005·British Journal of Haematology·G StussiJ D Seebach
Mar 26, 2013·American Journal of Clinical Pathology·Marco LaiRaffaele Landolfi
Dec 10, 1999·Cytometry·G Garratty, P A Arndt

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