Flow cytometric analysis of BCL-2 can distinguish small numbers of acute lymphoblastic leukaemia cells from B-cell precursors

British Journal of Haematology
Leah Hartung, David W Bahler

Abstract

Flow cytometric identification of small numbers of precursor B-cell acute lymphoblastic leukaemia (B-ALL) cells in post-treatment marrow specimens could benefit from the identification of additional, easily detectable markers that could be used in most cases. In this study, we evaluate whether bcl-2 expression quantified by four-colour flow cytometry can be effectively used to discriminate precursor B-ALL blasts from normal B-cell precursors (haematogones) and function as a leukaemia-specific marker. Levels of bcl-2 in the 22 precursor B-ALL cases studied were found to be significantly higher (over sixfold higher on average) than those present in haematogone populations from 22 control marrow specimens. Higher relative levels of bcl-2 expression in the B-ALL cases were maintained with respect to both immature CD34+ and more mature CD34- haematogone subsets, and appeared stable. Dilutional studies indicated that multiparameter flow cytometry analysis using bcl-2 could identify precursor B-ALL blasts representing as few as 1% of CD19+ cells or 0.01% of total leucocytes in bone marrow specimens containing substantial numbers of haematogones. This study suggests that bcl-2 may be an important marker for flow cytometric detection an...Continue Reading

References

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Jan 11, 2003·Archives of Pathology & Laboratory Medicine·Zahid KaleemGlenda White
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Jun 6, 2003·British Journal of Haematology·Dario Campana

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Citations

Jan 17, 2008·Blood·Fiona E Craig, Kenneth A Foon
Dec 17, 2009·Leukemia & Lymphoma·Deborah W SevillaBachir Alobeid
Mar 10, 2005·British Journal of Haematology·Lawrence TsaoBachir Alobeid
Oct 28, 2011·Cytometry. Part B, Clinical Cytometry·Fiona E CraigSteven H Swerdlow
Aug 13, 2013·Leukemia Research·S P ChantepieO Reman
Jul 10, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L Y ShiuK W Kuo
Jul 31, 2007·Clinics in Laboratory Medicine·Fiona E Craig

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