Flow cytometric analysis of BrdU incorporation as a high-throughput method for measuring adult neurogenesis in the mouse.

Journal of Pharmacological and Toxicological Methods
Darrick T BaluIrwin Lucki

Abstract

The generation of new neurons occurs throughout adulthood in discrete brain regions, and may be regulated by neuropsychiatric diseases and therapeutic drug treatments. Most current methods that study this process measure the labeling of newborn cells by 5-bromo-2-deoxyuridine (BrdU) using immunohistochemical methods followed by the microscopic counting of BrdU positive cells. This method is time consuming and labor intensive, typically taking several weeks to analyze. Therefore, we characterized a method to measure BrdU incorporation in the adult mouse hippocampus in vivo by using flow cytometry, which normally allows analysis of data within a single day. The present study compared multiple BrdU dosing and loading protocols to determine a dosing strategy that produced the best signal to noise ratio. BrdU incorporation was also compared across different brain regions. The method was sensitive to a number of experimental disease manipulations. Induction of type-1 diabetes and depletion of norepinephrine reduced hippocampal cell proliferation. In contrast, chronic administration of electroconvulsive shock, a somatic treatment for depression, as well as chronic treatment with the antidepressant fluoxetine elevated hippocampal cell ...Continue Reading

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Citations

Oct 16, 2009·The Journal of Pharmacology and Experimental Therapeutics·Georgia E HodesIrwin Lucki
May 30, 2013·Development·Daniel A BergAndrás Simon
Jul 22, 2009·Experimental Brain Research·Michael J ColditzElizabeth J Coulson
Apr 13, 2010·Neurobiology of Disease·Chad E BeyerGarth T Whiteside
Nov 26, 2009·European Journal of Pharmacology·Briony J CatlowJuan Sanchez-Ramos
Aug 5, 2011·Journal of Neurochemistry·Robert SpoelgenArmin Schneider
May 18, 2013·Neuroscience and Biobehavioral Reviews·Nancy HoIrwin Lucki
Sep 29, 2011·Physiology & Behavior·Nancy HoIrwin Lucki
Jun 16, 2015·BioMed Research International·Shin FujimakiTomoko Kuwabara
Jun 15, 2012·The International Journal of Neuropsychopharmacology·Ning SongTsukasa Koyama
Oct 19, 2017·International Journal of Molecular Sciences·Shin Fujimaki, Tomoko Kuwabara
Jan 30, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Darrick T BaluIrwin Lucki
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Akinobu KameiPeter J Murray
Mar 11, 2018·Advanced Drug Delivery Reviews·Ellen Heber-Katz, Phillip Messersmith

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