Flow cytometric analysis of extracellular vesicle subsets in plasma: impact of swarm by particles of non-interest

Journal of Thrombosis and Haemostasis : JTH
S F W M LibregtsM H M Wauben

Abstract

Essentials Extracellular vesicles (EVs) in biological fluids are promising biomarkers for disease. Fluorescence-based flow cytometric analysis is suitable to detect low abundant EV subsets. Particles of non-interest can induce false-positive light scatter and fluorescent signals. Interference of particles of non-interest can be monitored by analyzing serial dilutions. Background Extracellular vesicles (EVs) in plasma are increasingly being recognized as potential biomarkers. EV analysis for diagnostic purposes should be robust and should allow analysis of EV subsets with a wide range of abundance and in a large number of patient samples. Flow cytometry offers possibilities to meet these criteria, as it allows multiparameter analysis of individual EVs. However, analysis of plasma EVs is challenging, because of their size and heterogeneity, and the presence of other submicrometer-sized particles in plasma that could interfere with EV analysis. Objectives To explore whether fluorescence-based flow cytometric analysis of EV subsets is suitable when the EVs of interest are present in low abundance in a background of non-labeled or differently labeled EVs and particles. Methods Fluorescently labeled EVs of interest were spiked at dif...Continue Reading

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Citations

Apr 6, 2019·Journal of Extracellular Vesicles·Lisa AyersRienk Nieuwland
Mar 21, 2019·Cellular and Molecular Life Sciences : CMLS·Marta Monguió-TortajadaFrancesc E Borràs
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Mar 14, 2019·Journal of Extracellular Vesicles·Estefanía Lozano-AndrésMaría Yáñez-Mó
Dec 19, 2018·Neuro-oncology·Kyle FraserRalph Weissleder
Mar 5, 2020·Journal of Extracellular Vesicles·Joshua A WelshJennifer C Jones
Jun 6, 2019·Journal of Extracellular Vesicles·Kenneth W WitwerHidetoshi Tahara
Sep 19, 2020·Journal of Extracellular Vesicles·Tom A P DriedonksEsther N M Nolte-'t Hoen
Sep 24, 2020·American Journal of Reproductive Immunology : AJRI·Martine KuiperEdwin van der Pol
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Joana MaiaBruno Costa-Silva
Jan 26, 2021·Molecular Biotechnology·Talitha Keren KurianNirmal Mazumder
Dec 31, 2020·International Journal of Molecular Sciences·Marco MarchisioPaola Lanuti
Oct 30, 2020·International Journal of Molecular Sciences·Pasquale SimeonePaola Lanuti
Sep 12, 2020·Molecular Therapy. Methods & Clinical Development·Laetitia S GasparLuís Pereira de Almeida
May 1, 2021·International Journal of Molecular Sciences·Sobha Karuthedom GeorgeViktoria Weber
May 4, 2021·Journal of Extracellular Vesicles·Silvia OggeroMauro Perretti

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