Flow cytometric analysis of intestinal intraepithelial lymphocytes in the diagnosis of refractory celiac sprue

European Journal of Gastroenterology & Hepatology
Laura B Sanchez-MuñozG Roy

Abstract

The etiology of refractory celiac sprue (RCS) is unclear. In a high proportion of cases, the clonal nature of intestinal intraepithelial lymphocytes (IEL) can be demonstrated and a pathogenetic implication of intestinal IEL has been postulated. The prognosis of this subgroup of RCS is poor, with a high risk to develop an overt lymphoma and uncontrolled malabsorption despite steroid/immunosuppressive therapy. Cases with a relatively indolent clinical course, however, exist and their early diagnosis may be difficult. To gain insight into the pathogenic implication of intestinal IEL in refractory celiac sprue, we have performed an extensive phenotypic and functional characterization of clonal intestinal IEL in a patient with an indolent form of refractory celiac sprue, using multiparametric flow cytometry. The abnormal lymphocyte infiltrate lacked surface membrane expression of CD3/T-cell receptor (TCR) complexes (TCR(-), CD4(-), CD8(-), sCD3(-)), but contained intracellular CD3(epsilon) (CyCD3(+)) and surface CD103(+) and CD7(+). In particular, these cells showed a unique spontaneous ex-vivo cytokine secretion profile with an increased percentage of CD3(-) IEL containing TNF-alpha and IL-10, in the absence of IL-2, IL-4 and IFN-g...Continue Reading

References

Aug 1, 1994·Journal of Clinical Pathology·R G ShidrawiP J Ciclitira
Oct 1, 1996·Alimentary Pharmacology & Therapeutics·S O'MahonyM S Losowsky
Jul 15, 1999·Scandinavian Journal of Immunology·H K SillettL K Trejdosiewicz
Jul 13, 2000·Gastroenterology·B M Ryan, D Kelleher
Nov 17, 2001·European Journal of Gastroenterology & Hepatology·C J MulderE Metselaar
Mar 5, 2002·Gastroenterology·Helen R GillettSubrata Ghosh
Aug 23, 2002·The American Journal of Gastroenterology·Ahmad S AbdulkarimJoseph A Murray
Sep 19, 2002·Revista clínica española·P EirasG Roy
Dec 11, 2002·Scandinavian Journal of Immunology·R W OlaussenI N Farstad
Aug 2, 2003·Gastroenterology·Francisco LeónGarbiñe Roy
Sep 3, 2003·Alimentary Pharmacology & Therapeutics·M S GoerresC J J Mulder
Apr 7, 2005·Digestive Diseases and Sciences·Francisco LeónGarbiñe Roy
May 10, 2005·European Journal of Gastroenterology & Hepatology·Sue M TurnerChris S J Probert
Jun 1, 2005·Best Practice & Research. Clinical Gastroenterology·Severin DaumChris J J Mulder
May 10, 2006·The Journal of Experimental Medicine·Bertrand MeresseBana Jabri
Jun 9, 2006·The New England Journal of Medicine·Santiago VivasDimas Suárez-Vilela
Jan 20, 2007·Digestive Diseases and Sciences·Cristina CamareroGarbiñe Roy

❮ Previous
Next ❯

Citations

Nov 25, 2011·Gastroenterología y hepatología·Victoria Busto BeaAna Cano Ruiz
Sep 14, 2010·Journal of Immunological Methods·Francisco Leon
Jan 10, 2017·Nutrients·María de Lourdes MorenoIsabel Comino
Apr 5, 2013·Therapeutic Advances in Chronic Disease·Jeremy Woodward
May 7, 2019·Scandinavian Journal of Gastroenterology·Raquel Ríos LeónAgustín Albillos
Jan 15, 2020·World Journal of Gastroenterology : WJG·Richard Pg Charlesworth
Feb 6, 2021·The American Journal of Surgical Pathology·Craig R SoderquistGovind Bhagat
Sep 1, 2021·Human Immunology·Anastasia GandiniElizabeth Mayne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.