PMID: 2118453Aug 1, 1990Paper

Flow cytometric analysis of lymphocyte subpopulations in the spleen and thymus of mice exposed to an acute immunosuppressive dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Environmental Research
Nancy I Kerkvliet, J A Brauner

Abstract

The objective of the present studies was to determine if acute exposure to an immunotoxic dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) produces alterations in the expression of lymphocyte surface markers as measured by multiparameter flow cytometry. The immunotoxicity of a single oral dose of TCDD was assessed by the anti-SRBC PFC response; an ED50 of 0.74 micrograms/kg was determined. Subpopulations in the spleen and thymus of C57B1/6 mice were analyzed 2 days following exposure to 2 micrograms/kg TCDD. In addition, splenic lymphocyte subsets were examined on Days 1-4 following SRBC challenge of mice treated with 0, 2, or 5 micrograms/kg TCDD. T and B cells were identified by single parameter analysis of Thy 1.2 and Ig expression. T cell subsets were defined by dual parameter analysis of CD4 and CD8 expression. In TCDD-treated mice, the percentage and the total number of double-positive CD4+ CD8+ thymocytes were significantly decreased while the percentage but not the total number of double-negative CD4- CD8- thymocytes was significantly increased. No changes in the percentage or total number of single positive (CD4+ CD8- or CD4- CD8+) thymocyte subsets were observed. In contrast to the thymus, lymphocyte subsets in the ...Continue Reading

References

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Citations

Nov 1, 1995·International Journal of Immunopharmacology·R A PrellN I Kerkvliet
Jan 1, 1991·Toxicology·J A Deyo, N I Kerkvliet
Aug 1, 1996·Risk Analysis : an Official Publication of the Society for Risk Analysis·L F McGrathM A Gallo
Mar 26, 2010·Environmental Health : a Global Access Science Source·Lenka L Allan, David H Sherr
Mar 1, 1995·Environmental Health Perspectives·L S Birnbaum
Dec 1, 1995·Environmental Health Perspectives·N I Kerkvliet
Nov 23, 2006·Annals of the New York Academy of Sciences·Rachel M Patterson, Dori R Germolec
Feb 1, 1992·Toxicology and Applied Pharmacology·B L BlaylockM I Luster
Feb 1, 1997·Toxicology and Applied Pharmacology·A B KamathM Nagarkatti
Feb 14, 2006·Biochemical and Biophysical Research Communications·Lenka L AllanDavid H Sherr
Jun 12, 2003·Archives of Toxicology·Kai RieckeRalf Stahlmann
Jan 15, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Deborah E KeilMargie M Peden-Adams
Aug 24, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stefanie ChmillCharlotte Esser
Nov 3, 2015·Current Protocols in Toxicology·A Graham PockleyGabriele Multhoff
Mar 1, 2005·Current Protocols in Toxicology·Julie A Oughton, Nancy I Kerkvliet
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shuhei TomitaYousuke Takahama
Oct 14, 2020·Nature Immunology·Takafumi SuzukiMasayuki Yamamoto
Dec 1, 1992·Toxicology and Applied Pharmacology·J A HardinD H Sherr

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