Flow cytometric analysis of rodent epididymal spermatozoal chromatin condensation and loss of free sulfhydryl groups.

Molecular Reproduction and Development
D P EvensonL K Jost

Abstract

Flow cytometric measurements were made on acridine orange (AO) and 7-diethylamino-3-(4'-maleimidylphenyl)-4-methyl-coumarin (CPM)-stained epididymal- and vas deferens-derived spermatozoal nuclei to follow the course of chromatin condensation and oxidation of free sulfhydryl groups, respectively, during passage through mouse and rat posttesticular reproductive tracts. Alterations of mouse and rat spermatozoal chromatin during transition from a testicular elongated spermatids to epididymal caput spermatozoa resulted in a threefold loss of DNA stainability with AO. Passage of spermatozoa from the caput to corpus epididymis was accompanied by an approximate 15% loss of DNA stainability, which was maintained at that level throughout passage into the vas deferens. AO stainability of epididymal spermatozoal nuclei was generally independent of -SH group stainability. CPM stainability of rat spermatozoal nuclei free -SH groups was 83%, 18%, and 11% of caput spermatozoal values for corpus, cauda epididymis, and vas deferens, respectively. Comparable values for mice were 69%, 20%, and 18%. CPM stainability was relatively homogeneous for these mouse and rat reproductive tract regions, except mouse corpus epididymis spermatozoal nuclei stai...Continue Reading

References

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Citations

Jul 1, 1997·Molecular Reproduction and Development·J SeligmanR Shalgi
Jan 5, 2000·Molecular Reproduction and Development·R GolanL M Lewin
Jan 1, 1994·Molecular Reproduction and Development·S YossefiL M Lewin
Jan 1, 1993·Biology of the Cell·M Spanò, D P Evenson
Oct 4, 2005·Reproductive Toxicology·I Hernández-OchoaB Quintanilla-Vega
Mar 1, 2005·Molecular Reproduction and Development·Liga E Bennetts, R John Aitken
Sep 5, 2008·Current Protocols in Cytometry·D Evenson, L Jost
Jan 1, 1993·Environmental and Molecular Mutagenesis·D P EvensonR K Baer
Feb 17, 2005·Journal of Andrology·Klaus StegerMartin Bergmann
Oct 13, 2006·Reproduction, Fertility, and Development·Rebecca E SpindlerDavid E Wildt
Dec 26, 2001·Archives of Medical Research·M R Sairam, H Krishnamurthy

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