Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides

Journal of Immunology Research
Serkan YazıcıŞükran Tunali

Abstract

We retrospectively analyzed the clinicopathological correlation and prognostic value of cell surface antigens expressed by peripheral blood mononuclear cells in patients with mycosis fungoides (MF). 121 consecutive MF patients were included in this study. All patients had peripheral blood flow cytometry as part of their first visit. TNMB and histopathological staging of the cases were retrospectively performed in accordance with International Society for Cutaneous Lymphomas/European Organization of Research and Treatment of Cancer (ISCL/EORTC) criteria at the time of flow cytometry sampling. To determine prognostic value of cell surface antigens, cases were divided into two groups as stable and progressive disease. 17 flow cytometric analyses of 17 parapsoriasis (PP) and 11 analyses of 11 benign erythrodermic patients were included as control groups. Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3(+), CD4(+), CD8(+), TCRαβ(+), TCRγδ(+), CD7(+), CD4(+)CD7(+), CD4(+)CD7(-), and CD71(+)), B cells (HLA-DR(+), CD19(+), and HLA-DR(+)CD19(+)), NKT cells (CD3(+)CD16(+)CD56(+)), and NK cells (CD3(-)CD16(+)CD56(+)). The mean value of all cell surface antigens was not statistically signifi...Continue Reading

References

Jan 1, 1984·Cancer Immunology, Immunotherapy : CII·S LevyJ M Lang
Dec 17, 2003·The Journal of Investigative Dermatology·Hong WuTahseen Al-Saleem
Dec 19, 2006·Journal of Immunological Methods·Heddy ZolaXifeng Yang
Aug 21, 2007·Journal of the American Academy of Dermatology·Shaheen OshtoryHoward J Meyerson
Jun 11, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Adriana Garcia-HerreraElias Campo
Mar 17, 2009·Acta Haematologica·Satoshi HaraToshiaki Hanafusa
Mar 26, 2009·The American Journal of Dermatopathology·Nicole N BalmerAleodor A Andea
Nov 26, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Bo FengSa A Wang
Oct 21, 2010·American Journal of Clinical Pathology·Katalin KelemenGuang Fan
May 10, 2011·The American Journal of Dermatopathology·Chris H JokinenHarvey A Greisman
May 18, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Elise A OlsenUNKNOWN Cutaneous Lymphoma Task Force of the European Organisation for Research and Treatment of Cancer
Nov 19, 2011·American Journal of Clinical Pathology·Alexandra C HristovMichael J Borowitz
Oct 23, 2013·The American Journal of Surgical Pathology·Marie-Laure JulliéBéatrice Vergier
Jul 22, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Anna E S Brooks

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Methods Mentioned

BETA
flow cytometry
flow
biopsy

Software Mentioned

SPSS

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