PMID: 222110Apr 12, 1979Paper

Flow cytometric analysis of the DNA distribution in human brain tumors

Acta Neuropathologica
K KawamotoL G Koss

Abstract

Flow cytofluorometric analysis was used to determine the distribution of the DNA content in cells from selected areas of normal human brain and in benign and malignant brain tumors. Propidium iodide was employed as DNA fluorochrome and the analysis was carried out on a suspension of single cells. Normal, nonstimulated human lymphocytes were used as diploid controls. With nonneoplastic tissue an average of 91% of the cells were diploid (presumably in G0 or G1 stage of the cell cycle). The cells of most benign tumors were mainly diploid (77-98%), nine specimens of pituitary adenomas had large numbers of aneuploid cells. In glioblastoma multiforme the proportion of diploid cells was significantly diminished and polyploid cells were frequently seen. Similar results were obtained in other malignant tumors, with metastatic tumors showing the greatest ploidy variation, which included triploid, tetraploid, and hypertetraploid cells. The analytical method used provides valuable information of significant clinical importance on the DNA distribution in brain tumor cells.

References

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Citations

Mar 1, 1989·Journal of Neurosurgery·M YasueK D Bauer
Apr 15, 1984·Cancer·M AnnikoJ Wersäll
Oct 3, 1999·Pediatric Hematology and Oncology·M W Ben ArushS Postovski
Feb 4, 2005·Journal of Neuro-oncology·Basil F El-RayesJohn F Ensley
Apr 1, 1992·Cancer·J M KrosT H van der Kwast
Jan 1, 1990·Neurosurgical Review·F W SpaarE Markakis
Jan 1, 1983·Archives of Oto-rhino-laryngology·M AnnikoJ Wersäll
Jan 1, 1982·Acta Neuropathologica·H Martin, K Voss
Jan 1, 1980·Acta Neuropathologica·J Lehmann, H Krug
Oct 1, 1995·Journal of Neuro-oncology·Y YoshiiT Nose
Aug 15, 2012·The Journal of Cell Biology·Amy B ForakerFrances M Brodsky
Jul 1, 1981·Cytometry·O D Laerum, T Farsund
Aug 1, 1980·Journal of Neurosurgery·S J Mørk, O D Laerum
Mar 1, 1980·Histopathology·J De ReuckH V Eecken
Jan 1, 1989·Acta neurochirurgica·K KawamotoH Matsumura
Jan 1, 1980·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K KawamotoL G Koss
Feb 4, 2021·Methods and Protocols·Evrysthenis VartholomatosGeorgios S Markopoulos
Feb 23, 2020·Clinical Neurology and Neurosurgery·George A AlexiouAthanasios P Kyritsis

Related Concepts

Aneuploid Cell
Brain Tumor, Recurrent
Diploid Cell
DNA, Neoplasm
Glioblastoma Multiforme
Intracranial Meningeal Neoplasms
Spinal Meningioma

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