PMID: 11335135May 4, 2001Paper

Flow cytometric analysis of the localization of Helicobacter pylori antigens during different growth phases

FEMS Immunology and Medical Microbiology
K BlomA Svennerholm

Abstract

Previous studies on the localization of several different Helicobacter pylori antigens have been contradictory. We have therefore examined by using both one- and two-color flow cytometry (FCM), immunofluorescence (IF), and immunoelectron microscopy (IEM), the possible surface localization of some H. pylori antigens that may be important virulence factors. All four methods detected the lipopolysaccharide and the N-acetyl-neuroaminyllactose-binding hemagglutinin protein (HpaA) as surface-exposed, while the urease enzyme was not detected at all and the neutrophil activating protein only in low concentration on the surface of the H. pylori bacteria during culture of H. pylori in liquid broth for 11 days. The FCM analysis was found to be quite sensitive and specific and also extremely fast compared with IF and IEM, and therefore the preferred method for detection of surface-localized antigens of H. pylori.

References

Jan 11, 1991·Microbial Pathogenesis·D J EvansD Y Graham
Jan 1, 1980·Methods of Biochemical Analysis·E A Bayer, M Wilchek
Jun 1, 1995·Infection and Immunity·D J EvansP R Kvietys
Sep 1, 1994·The American Journal of Medicine·R J Hopkins, J G Morris
Nov 1, 1993·Medical Microbiology and Immunology·G BodeH Ditschuneit
Jul 25, 1997·The Journal of Biological Chemistry·S TenebergK A Karlsson
Jul 1, 1997·Journal of Applied Microbiology·E J Walsh, A P Moran

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Citations

May 20, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Anneli M LundstromSusanne Nystrom
Oct 13, 2006·Infection and Immunity·Ge WangRobert J Maier
Feb 24, 2004·Infection and Immunity·Niamh RocheSusann Teneberg
Jan 24, 2006·Infection and Immunity·Elisabet CarlsohnAnn-Mari Svennerholm
Feb 24, 2018·Virulence·John BenktanderSusann Teneberg
Jun 15, 2007·FEMS Immunology and Medical Microbiology·Philip SuttonAdrian Lee
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lee-Ann H AllenLara M Wittine
Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anna Konovalova, Thomas J Silhavy
Mar 10, 2007·Cellular Microbiology·Lee-Ann H Allen
Jul 8, 2005·FEMS Microbiology Letters·Mikael BrisslertMarianne Quiding-Järbrink

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