Flow cytometric analysis of the uptake of Hoechst 33342 dye by human lymphocytes

Cytometry
G SzabóS Damjanovich

Abstract

Human peripheral blood lymphocytes have been shown to resist staining with the DNA binding fluorochrome Hoechst 33342 by the cellular membrane. The rate of uptake of the dye is strongly temperature-dependent with minimal uptake rate below 16 degrees C. The activation energy of dye transport was found to be 135 kJ/mol above 20 degrees C and about 20 kJ/mol below 16 degrees C. Metabolic inhibitors accelerated, instead of inhibiting, the transport of the dye. Dead cells have been shown to stain promptly in contrast with the gradually staining viable cells. The uptake process in the vital staining conditions is suggested to involve a carrier mediated mechanism. Application of Hoechst 33342 as a fluorescent indicator of viability is proposed.

References

Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D J Arndt-Jovin, T M Jovin
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M E Lalande, R G Miller
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R G Langlois, R H Jensen
Apr 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R J HowardG F Mitchell
Jan 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M R Loken

❮ Previous
Next ❯

Citations

Jun 2, 1983·Biochimica Et Biophysica Acta·M Sasvári-SzékelyF Antoni
Jan 1, 1984·Cancer Investigation·F Traganos
Apr 18, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hong ZhaoZbigniew Darzynkiewicz
Jan 26, 2020·Scientific Reports·Erfaneh Firouzi NiakiGábor Szabó
Dec 28, 1984·Biochemical and Biophysical Research Communications·M Sasvari-SzekelyF Antoni

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.