Flow cytometric analysis to detect pathogens in bacterial cell mixtures using semiconductor quantum dots

Analytical Chemistry
Megan A HahnTodd D Krauss

Abstract

Compared to a common green organic dye, semiconductor quantum dots (QDs) composed of CdSe/ZnS core/shell bioconjugates display brighter fluorescence intensities, lower detection thresholds, and better accuracy in analyzing bacterial cell mixtures composed of pathogenic E. coli O157:H7 and harmless E. coli DH5alpha using flow cytometry. For the same given bacterial mixture, QDs display fluorescence intensity levels that are approximately 1 order of magnitude brighter compared to the analogous experiments that utilize the standard dye fluorescein isothiocyanate. Detection limits are lowest when QDs are used as the fluorophore label for the pathogenic E. coli O157:H7 serotype: limits of 1% O157:H7 in 99% DH5alpha result, corresponding to 106 cells/mL, which is comparable to other developing fluorescence-based techniques for pathogen detection. Finally, utilizing QDs to label E. coli O157:H7 in cell mixtures results in greater accuracy and more closely approaches the ideal fluorophore for pathogen detection using flow cytometry. With their broader absorption spectra and narrower emission spectra than organic dyes, QDs can make vast improvements in the field of flow cytometry, where single-source excitation and simultaneous detectio...Continue Reading

References

Apr 2, 1999·Applied and Environmental Microbiology·L GoodridgeM Griffiths
Sep 23, 2000·Journal of Microbiological Methods·H B Steen
Mar 1, 2003·Journal of Microbiological Methods·J A BoschA V Nieuw Amerongen
Jul 20, 1964·Archives of Biochemistry and Biophysics·L CHAIET, F J WOLF
Feb 14, 2004·Journal of Applied Microbiology·A K Deisingh, M Thompson
Jul 25, 2006·Nature Medicine·Pratip K ChattopadhyayMario Roederer

❮ Previous
Next ❯

Citations

Aug 10, 2010·Analytical and Bioanalytical Chemistry·Maureen A WallingJason R E Shepard
Apr 1, 2009·International Journal of Molecular Sciences·Maureen A WallingJason R E Shepard
Dec 24, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Siddhesh B ShindeVandana B Patravale
Jan 10, 2012·Enzyme and Microbial Technology·Niamh Gilmartin, Richard O'Kennedy
Dec 17, 2009·Biotechnology Advances·Vijayalakshmi VelusamyCatherine Adley
Mar 14, 2012·International Journal of Cancer. Journal International Du Cancer·Pericles PericleousEfstathios P Efstathopoulos
Jul 28, 2009·Biotechnology and Bioengineering·Peter B YimJeeseong Hwang
Jan 27, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Tymish Y OhulchanskyyParas N Prasad
Jan 27, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Pratip K ChattopadhyayMario Roederer
Aug 30, 2011·Analytical Biochemistry·Michael D LeipoldMark Nitz
Apr 29, 2015·The Analyst·Sukhendu NandiRaz Jelinek
Oct 4, 2014·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Gongping WangXiaoshan Feng
May 26, 2010·The Analyst·Krista Rule Wigginton, Peter John Vikesland
Oct 1, 2010·Critical Reviews in Microbiology·Jacques TheronMichele de Kwaadsteniet
Sep 17, 2013·Measurement Science & Technology·Ilaria AltamoreEnrico Gratton
Apr 25, 2013·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Varun K A SreenivasanEwa M Goldys
Sep 27, 2018·Journal of Food and Drug Analysis·Vivek K BajpaiYoung-Kyu Han
Apr 14, 2011·Chemical Society Reviews·Maureen A Walling, Jason R E Shepard
Jul 2, 2021·International Journal of Biological Macromolecules·Cínthia das Dores AguiarLuis Henrique Mendes da Silva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.