PMID: 9551607Apr 29, 1998Paper

Flow cytometric assessment of cell structural and functional changes induced by acetic acid in the yeasts Zygosaccharomyces bailii and Saccharomyces cerevisiae

Cytometry
C PrudêncioM Côrte-Real

Abstract

Flow cytometry (FCM) was used with different viability dyes to assess changes in cell structure and function induced by acetic acid (AA) in populations of Zygosaccharomyces bailii (AA resistant) and Saccharomyces cerevisiae (AA sensitive). Kinetic changes in esterase activity, intracellular dye processing, and membrane integrity were monitored, and to detect those changes we used three assays involving fluorescein diacetate hydrolysis, FUN-1 processing, and propidium iodide exclusion, respectively. In S. cerevisiae, the decrease in the ability to process FUN-1 preceded the decrease in esterase activity, and there was loss of cell membrane integrity after incubation with AA. In Z. bailii, with higher AA concentrations, there was a similar decrease in the ability to process FUN-1, which also preceded the loss of cell membrane integrity. Changes in esterase activity in this yeast induced by AA treatment could not be monitored because the changes occurred independently of the presence of the acid. For control samples (untreated cells killed with 10% v/v of AA), the percentages of nonaltered cells as estimated by FCM and percentages of viable cells as estimated by colony forming unit (CFU) counts were identical. However, for cell sa...Continue Reading

Citations

Sep 1, 2000·Infectious Diseases in Obstetrics and Gynecology·C Pina-VazP A Mårdh
Aug 1, 2002·Journal of Clinical Microbiology·S Arunmozhi Balajee, Kieren A Marr
Apr 28, 2011·Cell Biology International·Magdalena Kwolek-MirekGrzegorz Bartosz
Dec 12, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C Pina-VazJ Martinez-de-Oliveira
May 31, 2017·Yeast·Nurzhan KuanyshevPaola Branduardi
Sep 5, 2008·Current Protocols in Cytometry·Hazel M DaveyArseny S Kaprelyants
Oct 27, 2004·FEMS Microbiology Letters·Cecília Alves-AraújoCecília Leão
Sep 16, 2014·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Ana Carolina Loureiro Gama MotaEdeltrudes de Oliveira Lima
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·Mahomed-Yunus S MoosaRobert A Akins
Jul 10, 2002·Annals of the New York Academy of Sciences·Cristina PrudêncioMaria Amélia Tavares
Jan 17, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gail B GnoinskiFiona L Kerslake
Jan 10, 2021·International Journal of Biological Macromolecules·Cátia Santos-PereiraManuela Côrte-Real
Jul 9, 2011·Mechanisms of Ageing and Development·Alejandro Ocampo, Antoni Barrientos

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