PMID: 11929494Apr 4, 2002Paper

Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to betalactam antibiotics

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
M L SanzA L De Weck

Abstract

In this study, we used flow cytometry to determine the percentage of activated basophils that expressed the CD63 marker after in vitro stimulation by different betalactam antibiotics. The diagnostic reliability of the technique was assessed, as well as its correlation with specific IgE. Fifty-eight patients with clinical allergy to betalactam antibiotics and presenting positive skin tests to at least one of the allergens (minor determinant mixture (MDM), benzylpenicilloyl-polylysine (PPL), penicillin, ampicillin, amoxicillin, cephalosporins) were tested. Thirty subjects non-allergic to betalactams were also studied as controls. The flow assay stimulation test (FAST) uses flow cytometry to determine the percentage of basophils that express CD63 as an activation marker after in vitro stimulation with allergen. Double labelling with monoclonal antibodies anti-CD63-PE and anti-IgE FITC was used. The allergic patients show a statistically greater number of activated basophils than the control subjects, after the incubation of cells with all the betalactams at various concentrations. The sensitivity of the technique is 50%, the specificity 93.3%, the likelihood ratio for a positive value 7.46 and the likelihood ratio for a negative v...Continue Reading

References

Sep 1, 1991·The Journal of Allergy and Clinical Immunology·E F KnolD Roos
Sep 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M BlancaC Juarez
Sep 1, 1995·International Archives of Allergy and Immunology·F MorenoA Miranda
Aug 1, 1995·Cytometry·J SzöllösiF M Waldman
Nov 24, 1993·JAMA : the Journal of the American Medical Association·J GaddeN F Adkinson
Mar 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J Sainte-LaudyJ C Guerin
Jan 20, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A RomanoM Blanca
Nov 27, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J Sainte-LaudyJ C Guerin
Feb 13, 1999·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·D A Moneret-VautrinS Frémont
May 21, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B A Baldo
Feb 12, 2000·The Journal of Allergy and Clinical Immunology·A Pâris-KöhlerB Arnoux
Mar 22, 2000·Allergy·C I EzeamuzieZ Hijazi
Jul 27, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M L SanzA L De Weck

❮ Previous
Next ❯

Citations

Sep 28, 2010·Archivum Immunologiae Et Therapiae Experimentalis·Anna Wolanczyk-MedralaWojciech Medrala
Dec 31, 2004·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·S M ErdmannH F Merk
Jan 22, 2005·Current Allergy and Asthma Reports·Pascal Demoly, Antonino Romano
Jul 27, 2011·Clinical Reviews in Allergy & Immunology·Christopher ChangM Eric Gershwin
Apr 3, 2013·Clinical Reviews in Allergy & Immunology·Scott D Dickson, Kimberly C Salazar
Mar 11, 2004·Veterinary Immunology and Immunopathology·Yumiko SakuraiHiroomi Akashi
Jan 13, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Esther A CoorsVera Mahler
Jul 9, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Bernard Yu-Hor Thong, Yeow-Chan
Dec 14, 2004·International Archives of Allergy and Immunology·Marie-Laure HemeryPascal Demoly
Jul 14, 2006·International Archives of Allergy and Immunology·Jörg Kleine-TebbeBernhard F Gibbs
Feb 13, 2008·International Archives of Allergy and Immunology·A L de WeckJ Sainte-Laudy
Feb 22, 2008·AJR. American Journal of Roentgenology·Jiri TrckaAxel Trautmann
Jul 22, 2009·AJR. American Journal of Roentgenology·Urs C SteinerWerner J Pichler
Nov 22, 2013·Asia Pacific Allergy·Woo-Jung Song, Yoon-Seok Chang
Mar 12, 2011·Annales Françaises D'anesthèsie Et De Rèanimation·L GuillouxE Beaudouin
Apr 8, 2014·Mediators of Inflammation·Ji-Fu WeiShaoheng He
Nov 1, 2006·Expert Review of Clinical Immunology·Andreas Beeler, Werner J Pichler
May 21, 2011·Expert Review of Clinical Immunology·Julie LeysenDidier G Ebo
Dec 24, 2005·Expert Opinion on Drug Safety·Rebeca Rodriguez-PenaMaria Jose Torres
May 9, 2008·Expert Opinion on Drug Safety·Esther MorenoFélix Lorente
Dec 15, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M J TorresM Blanca
Oct 1, 2004·Allergologia et immunopathologia·O Cáceres Calle, M Fernández-Benítez
Feb 15, 2011·The Journal of Allergy and Clinical Immunology·Marta FerrerAllen P Kaplan
Oct 12, 2010·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·UNKNOWN Joint Task Force on Practice ParametersUNKNOWN Joint Council of Allergy, Asthma and Immunology
Jul 1, 2009·Immunology and Allergy Clinics of North America·Oliver V HausmannDidier G Ebo
Sep 4, 2007·The Journal of Allergy and Clinical Immunology·Shabnam ForoughiCalman Prussin
Sep 17, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A RomanoJ-L Guéant
Nov 17, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M J TorresM Blanca
Sep 7, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S M ErdmannH Merk
Sep 7, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P GamboaA L de Weck
Mar 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D G EboW J Stevens
Jul 16, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P S FriedmannR St C Barnetson
May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S M ErdmannB Sachs
Apr 30, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E GómezC Mayorga
Aug 13, 2003·Allergy·W AbererUNKNOWN EAACI interest group on drug hypersensitivity
Sep 27, 2003·Allergy·M J TorresUNKNOWN EAACI Interest Group on Drug Hypersensitivity

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.