PMID: 8603109Jan 1, 1996Paper

Flow cytometric BrdUrd-pulse-chase study of X-ray-induced alterations in cell cycle progression

Cell Proliferation
R HigashikuboJ L Roti Roti

Abstract

To better understand how the flow cytometric bromodeoxyuridine (BrdUrd) -pulse-chase method detects perturbed cell kinetics we applied it to measure cell cycle progression delays following exposure to ionizing radiation. Since this method will allow both the use of asynchronous cell populations and the determination of the alterations in cell cycle progression specific to cells irradiated in given cell cycle phases, it has a significant advantage over laborious synchronization methods. Exponentially growing Chinese hamster ovary (CHO) K1 cells were irradiated with graded doses of X-rays and pulse-labelled with BrdUrd immediately thereafter. Cells were subcultured in a BrdUrd-free medium for various time intervals and prepared for flow cytometric analysis. Of five flow cytometric parameters examined, only those that involved cell transit through G2, i.e., the fraction of BrdUrd-negative G2 cells and the fraction of BrdUrd-positive cells that had not divided, showed radiation dose-dependent delays. The magnitude of the effects indicates that the cells irradiated in G2 and in S are equally delayed. S phase transit of cells irradiated in S or in G1 did not appear to be affected. There were apparent changes in flow of cells out of G...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J KuerbitzM B Kastan
Jan 1, 1966·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·G Froese
Mar 1, 1980·Cell and Tissue Kinetics·S P TomasovicL A Dethlefsen
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Jun 1, 1959·Experimental Cell Research·H QUASTLER, F G SHERMAN
Aug 1, 1961·Proceedings of the National Academy of Sciences of the United States of America·M YAMADA, T T PUCK

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Citations

Apr 24, 2001·International Journal of Radiation Oncology, Biology, Physics·D BartkowiakE M Röttinger
May 31, 2001·International Journal of Radiation Oncology, Biology, Physics·N A ScottK K Griendling
Mar 18, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Stephen B Keysar, Michael H Fox
Aug 8, 2015·Radiation Oncology·Arlene L OeiPrzemek M Krawczyk

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