Flow Cytometric Characterization of S-phase Fraction and Ploidy in Lymph Node Aspirates from Dogs with Lymphoma

Journal of Comparative Pathology
B MiniscalcoF Riondato

Abstract

Canine lymphoma is a multifaceted disease encompassing numerous entities with different prognosis. Objective assessment of the proliferation rate is of importance from the pathological and clinical perspectives. Different methods have been described in the literature to assess proliferation rate, including evaluation of Ki67 expression in fresh lymph node (LN) aspirates measured by flow cytometry (FC). This test has a high accuracy in discriminating between low- and high-grade lymphomas, and provides prognostic information among high-grade B-cell lymphomas. DNA content analysis is less expensive and suitable for well-preserved samples. We describe DNA-content analysis using LN aspirates from 112 dogs with lymphoma. S-phase fraction (SPF) accurately discriminated between low- and high-grade lymphomas, with 3.15% being the best discriminating cut-off value. SPF values strongly correlated with Ki67 expression as assessed by FC. Survival analyses were restricted to 33 dogs with high-grade B-cell lymphoma receiving standardized multi-agent chemotherapy, but no significant result was obtained for SPF. We also describe a subset of aneuploid cases and their respective follow-up. We conclude that DNA content analysis may be combined wit...Continue Reading

Citations

Dec 18, 2019·BMC Veterinary Research·Amadeo M ParissentiBrenda L Coomber
Mar 27, 2021·Veterinary Pathology·Giancarlo AvallonePaola Roccabianca
Mar 30, 2021·Research in Veterinary Science·Fulvio RiondatoBarbara Miniscalco
Mar 25, 2021·Veterinary and Comparative Oncology·Antonella RigilloBarbara C Rütgen
Apr 20, 2021·Frontiers in Veterinary Science·Fulvio Riondato, Stefano Comazzi
May 11, 2021·Frontiers in Veterinary Science·Stefano Comazzi, Fulvio Riondato

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.