Flow cytometric detection of cell-associated interleukin-8 in alveolar macrophages in vivo from patients with hypersensitivity pneumonitis and sarcoidosis

Scandinavian Journal of Clinical and Laboratory Investigation
S FujishimaK Yamaguchi

Abstract

In comparison with neutrophil-mediated lung diseases, such as acute respiratory distress syndrome, the involvement of IL-8 in lymphocyte-mediated lung diseases has not been fully investigated. Several reports have shown a slight increase in bronchoalveolar lavage fluid (BALF) IL-8 in patients with hypersensitivity pneumonitis (HP) and sarcoidosis (SAR), but the source of the IL-8 has not been clarified. In the present study, the in vivo production of IL-8 by alveolar macrophages (AMs) is examined in these patients by analyzing the cell-associated IL-8, using the flow cytometric method adopted previously. The IL-8 levels in the epithelial lining fluid (ELF) were also assessed. Initially, slight, but significant, increased levels of ELF IL-8 in HP and SAR were confirmed. Using flow cytometric analysis, a significant increase was found in the cell-associated IL-8 of the freshly isolated AMs in HP, but not in SAR, indicating in vivo production of IL-8 by AMs in HP. The cell-associated IL-8 of the AMs cultured with or without lipopolysaccharide was also analyzed. However, in contrast to previous findings in patients with idiopathic pulmonary fibrosis, no differences were found between SAR and HP patients and control subjects. Based ...Continue Reading

References

Nov 1, 1995·American Journal of Respiratory and Critical Care Medicine·H NakamuraA Ishizaka
Jul 1, 1995·The Journal of Clinical Investigation·H G FolkessonV C Broaddus
Jan 1, 1995·American Journal of Respiratory and Critical Care Medicine·M Denis
Jul 24, 1993·Lancet·S FujishimaN Aikawa
Mar 11, 2000·American Journal of Respiratory and Critical Care Medicine·M NakamuraK Yamaguchi

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Citations

Jan 2, 2009·Respiratory Medicine·Martina VasakovaIlja Striz
Jul 25, 2020·American Journal of Respiratory and Critical Care Medicine·Ganesh RaghuKevin C Wilson

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