Flow cytometric detection of erythrocyte osmotic fragility

Cytometry. Part B, Clinical Cytometry
Dong Il Won, Jang Soo Suh

Abstract

Hereditary spherocytosis (HS) is the most common cause of inherited chronic hemolysis. The various tests developed for HS screening have many shortcomings. The purpose of this study was to develop a new, simple, reliable test using flow cytometry. In this new test, deionized water, a hemolysis-inducing agent, is spiked to a red cell suspension during acquisition, and the count of red cells is measured sequentially in real-time using flow cytometry. In this study, the optimal parameters for this test were established, and the discriminatory power of the adopted protocol was verified among several groups with various degrees of osmotic fragility (OF). The established protocol successfully discriminated between blood analyzed immediately and blood stored for 24 h (P < 0.00001). With healthy individuals as a reference, the OF measured by this protocol was significantly decreased in patients with iron deficiency anemia (P = 0.030) and was significantly increased in HS patients (P = 1.1 x 10(-9)). This new test found all 11 HS patients to have increased OF. This new test was verified to be simple, quantitative, objective, and cost-effective for the measurement of OF. We suggest this test as another effective approach for HS screening...Continue Reading

References

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Citations

Aug 15, 2013·Indian Journal of Pediatrics·Hara Prasad Pati, Sonal Jain
Jul 27, 2015·Clinical Biochemistry·Mariela Granero Farias, Priscila Aparecida Correa Freitas
Apr 11, 2012·Pediatric Blood & Cancer·Renée L CrispHugo Donato
Feb 8, 2011·Cytometry. Part B, Clinical Cytometry·Prashant WarangRoshan Colah
Nov 19, 2013·Cytometry. Part B, Clinical Cytometry·Ye Jee Shim, Dong Ii Won
Dec 18, 2014·Cytometry. Part B, Clinical Cytometry·Rinkle MohapatraRoshan Colah
Jul 29, 2016·Annals of Hematology·Renée L CrispAlcira Nesse
Nov 13, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Mariela Granero Farias
Jan 20, 2017·Cytometry. Part B, Clinical Cytometry·Olga CiepielaIwona Kotuła
Sep 2, 2010·The Korean journal of laboratory medicine·Dong Il Won
Jan 28, 2014·Blood Research·Hye Lim Jung
Apr 23, 2014·Journal of Laboratory Automation·Ayako YamamotoHiroyuki Kayaba
Feb 8, 2019·International Journal of Laboratory Hematology·Estelle CheliFrancois Girodon
Aug 25, 2020·Journal of Korean Medical Science·Ye Jee ShimUNKNOWN Red Blood Cell Disorder Working Party of the Korean Society of Hematology
Dec 12, 2018·Cytometry. Part B, Clinical Cytometry·Kappelmayer Janos
May 27, 2015·Pediatrics·Robert D ChristensenPatrick G Gallagher

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