Flow cytometric detection of peripheral blood involvement by mycosis fungoides and Sézary syndrome using T-cell receptor Vbeta chain antibodies and its application in blood staging.

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Bo FengSa A Wang

Abstract

Peripheral blood involvement has been recognized as an adverse prognostic factor in patients with mycosis fungoides and Sézary syndrome. However, accurate identification and enumeration of the neoplastic cells in these diseases can be challenging. We assessed the clinical utility of flow cytometric immunophenotypic analysis of T-cell receptor Vbeta expression in 82 mycosis fungoides and 6 Sézary syndrome patients, with an atypical T-cell immunophenotype, or abnormal CD4:CD8 ratio, identified from peripheral blood specimens of 723 patients submitted for routine mycosis fungoides/Sézary syndrome blood staging. To improve detection sensitivity, Vbeta expression was analyzed on gated CD3+CD4+ T cells or T cells with an aberrant immunophenotype, if present. The flow cytometric results were compared with traditional morphologic assessment (n=88) and molecular methods to assess the T-cell receptor gamma or beta genes (n=41 tested in parallel). Flow cytometric immunophenotyping yielded a clonal Vbeta pattern in 60/82 mycosis fungoides and 6/6 Sézary syndrome patients. By contrast, flow cytometric Vbeta was negative in all 10 healthy donors and 18 control patients, showing a specificity of 100% and concordance with molecular testing of ...Continue Reading

References

Aug 1, 1978·The British Journal of Dermatology·S C Duncan, R K Winkelmann
Dec 1, 1988·Journal of the American Academy of Dermatology·E A AbelG S Wood
Aug 5, 2000·Cytometry·R van den BeemdJ J van Dongen
Feb 13, 2001·The British Journal of Dermatology·M G BernengoM T Fierro
Feb 24, 2001·Journal of the American Academy of Dermatology·G RapplU Reinhold
Jun 8, 2001·American Journal of Clinical Pathology·D JonesY O Huh
Sep 10, 2003·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·J Marcus MucheAnsgar Lukowsky
Aug 19, 1961·JAMA : the Journal of the American Medical Association·H F TASWELL, R K WINKELMANN
Mar 23, 2005·Archives of Dermatology·Robert Gniadecki, Ansgar Lukowsky
Jan 19, 2006·Blood·Nicolas OrtonneArmand Bensussan

❮ Previous
Next ❯

Citations

May 26, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Dimitri SalameireMarie-Christine Jacob
Dec 3, 2014·Photodiagnosis and Photodynamic Therapy·Manal BosseilaFatma Al-Helf
Oct 21, 2010·American Journal of Clinical Pathology·Katalin KelemenGuang Fan
Jan 21, 2016·Journal of Immunology Research·Serkan YazıcıŞükran Tunali
Dec 22, 2016·Expert Review of Hematology·Pooja VirmaniPierluigi Porcu
Nov 19, 2011·American Journal of Clinical Pathology·Alexandra C HristovMichael J Borowitz
Aug 2, 2018·Journal of Cellular Physiology·Saeed Mohammadian HaftcheshmehAmirhossein Sahebkar
Nov 11, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michaela KotrovaMonika Brüggemann
Dec 22, 2017·Oncotarget·Kamel LaribiAlix Baugier de Materre
Feb 13, 2016·The American Journal of Surgical Pathology·Mariko YabeRoberto N Miranda
Mar 4, 2015·Archives of Dermatological Research·Enrico ScalaMaria Grazia Narducci
Oct 14, 2020·The Journal of Investigative Dermatology·Pedro HornaHoratiu Olteanu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.