PMID: 9542916May 23, 1998Paper

Flow cytometric determination of ganciclovir susceptibilities of human cytomegalovirus clinical isolates

Journal of Clinical Microbiology
J J McSharryC S Crumpacker

Abstract

A flow cytometric assay has been developed for the measurement of susceptibilities to ganciclovir of laboratory strains and clinical isolates of human cytomegalovirus (HCMV). The assay uses fluorochrome-labeled monoclonal antibodies to HCMV immediate-early and late antigens to identify HCMV-infected cells and flow cytometry to detect and quantitate the number of antigen-positive cells. By this assay, the 50 and 90% inhibitory concentrations (IC50 and IC90, respectively) of ganciclovir for the AD169 strain of HCMV were 1.7 and 9.2 microM, respectively, and the IC50 for the ganciclovir-resistant D6/3/1 derivative of the AD169 strain was greater than 12 microM. The ganciclovir susceptibilities of 17 HCMV clinical isolates were also determined by flow cytometric analysis of the effect of ganciclovir on late-antigen synthesis in HCMV-infected cells. The average IC50 of ganciclovir for drug-sensitive HCMV clinical isolates was 3.79 microM (+/-2.60). The plaque-reduction assay for these clinical isolates yielded an average IC50 of 2.80 microM (+/-1.46). Comparison of the results of the flow cytometry assays with those obtained from the plaque-reduction assays demonstrated acceptable bias and precision. Flow cytometric and plaque-reduc...Continue Reading

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Citations

Nov 26, 1999·Antiviral Chemistry & Chemotherapy·A K Field
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·N S LurainUNKNOWN Adult AIDS Clinical Trials Group-CMV Laboratories
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·C Gilbert, G Boivin
Nov 5, 2002·Clinical and Diagnostic Laboratory Immunology·Christine ChutkowskiJames J McSharry
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·J J McSharryG L Drusano
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S Chou
Oct 31, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G Boivin
Nov 10, 2009·Future Microbiology·Henri AgutAgnès Gautheret-Dejean
May 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chae Hyun KimYoon-Jae Song
Sep 16, 1998·Antimicrobial Agents and Chemotherapy·J J McSharryC S Crumpacker
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·J J McSharry
Apr 9, 1999·Clinical Microbiology Reviews·A Erice
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez

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