Flow-cytometric determination of tumor cells in lymph nodes

Oncology
W ZoliD Amadori

Abstract

In solid tumors, metastasis occurs through the dissemination of tumor cells in the bloodstream and the lymphatic system. In particular, lymph node infiltration gives useful prognostic information and represents one of the most important factors for selecting the type of clinical treatment in disease management. Furthermore, the analysis of lymph node infiltration has become important for identifying patients with breast cancer or malignant melanoma who may be candidates for regional lymph node dissection. Tumor cells in lymph nodes are currently identified in tissue sections using morphological and immunohistochemical analyses, but these approaches are time-consuming, and micrometastases may escape detection. The aim of the present study was to define the potential of a flow cytometric (FCM) determination based on cell size and autofluorescence to shorten the time required for lymph node analysis. The sensitivity of the FCM approach, defined on mixtures of tumor cells from established cell lines and peripheral blood lymphocytes (PBL(s)) at different concentrations, was 1 tumor cell/1,000 PBL(s). FCM analysis was performed on 89 lymph nodes, 29 from breast, 41 from lung and 19 from colon cancer patients. Agreement between FCM an...Continue Reading

Citations

Mar 15, 2005·American Journal of Clinical Pathology·Ignacio CruzAlberto Orfao
Mar 23, 2011·The Journal of Urology·Jared M WhitsonMaxwell V Meng
Jan 29, 2014·International Journal of Cancer. Journal International Du Cancer·Marnie WinterBenjamin Thierry
Aug 21, 2004·Breast Cancer Research : BCR·Valérie ChoesmelNathalie Blin
Apr 2, 2008·Journal of Immunological Methods·Mona KarlssonOla Winqvist

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