Flow cytometric evaluation of ki67 for the determination of malignancy grade in canine lymphoma

Veterinary and Comparative Oncology
A PoggiF Riondato

Abstract

Ki67 is a nuclear antigen significantly correlated with degree of malignancy in human non-Hodgkin lymphomas. We wanted to assess the ability of flow cytometric evaluation of Ki67 index (Ki67I) in differentiating the grade of malignancy in canine lymphomas. Ki67I was determined on lymph node aspirates of 90 immunophenotyped lymphomas classified according to the updated Kiel classification: 80 high grade (HG, 62 B cell and 18 T cell) and 10 low grade (LG, 3 B cell and 7 T cell) lymphomas. HG lymphomas showed significantly higher Ki67I compared with LG lymphomas (P < 0.0001). A significant difference in HG lymphomas was detected between B- and T-immunophenotypes. Receiver operating characteristic (ROC) curve highlighted a high accuracy of Ki67I in recognizing HG lymphomas [area under the curve (AUC) = 99.4] and a cut-off value of 12.2% was established (sensitivity = 96.3% and specificity = 100%). Thus, we suggest the combination of Ki67I flow cytometric determination and immunophenotype as a reliable tool to classify canine lymphomas.

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Citations

Mar 10, 2016·The Veterinary Quarterly·M Zandvliet
Mar 26, 2016·Journal of Veterinary Internal Medicine·K MeichnerS E Suter
May 14, 2016·Cytometry. Part B, Clinical Cytometry·S ComazziUNKNOWN European Canine Lymphoma Network
Jan 23, 2016·Veterinary and Comparative Oncology·A PoggiF Riondato
Aug 4, 2016·Polish Journal of Veterinary Sciences·R SapierzyńskiD Jagielski
Mar 30, 2021·Research in Veterinary Science·Fulvio RiondatoBarbara Miniscalco
Mar 25, 2021·Veterinary and Comparative Oncology·Antonella RigilloBarbara C Rütgen
Apr 6, 2021·Frontiers in Veterinary Science·Luca AresuLaura Marconato
Apr 20, 2021·Frontiers in Veterinary Science·Fulvio Riondato, Stefano Comazzi
May 11, 2021·Frontiers in Veterinary Science·Stefano Comazzi, Fulvio Riondato

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