Flow cytometric gating for spleen monocyte and DC subsets: differences in autoimmune NOD mice and with acute inflammation

Journal of Immunological Methods
Matthew B DongKristin V Tarbell

Abstract

The role of antigen presenting cells (APCs) in the pathogenesis of autoimmune and other inflammatory diseases is now better understood due to advances in multicolor flow cytometry, gene expression analysis of APC populations, and functional correlation of mouse to human APC populations. A simple but informative nomenclature of conventional and plasmacytoid dendritic cell subsets (cDC1, cDC2, pDC) and monocyte-derived populations incorporates these advances, but accurate subset identification is critical. Ambiguous gating schemes and alterations of cell surface markers in inflammatory condition can make comparing results between studies difficult. Both acute inflammation, such as TLR-ligand stimulation, and chronic inflammation as found in mouse models of autoimmunity can alter DC subset gating. Here, we address these issues using in vivo CpG stimulation as an example of acute inflammation and the non-obese diabetic (NOD) mouse as a model of chronic inflammation.We provide a flow cytometric antibody panel and gating scheme that differentiate 2 monocytic and 3DC subsets in the spleen both at steady state and after CpG stimulation. Using this method, we observed differences in the composition of NOD DCs that have been previously r...Continue Reading

References

Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M C NussenzweigB Gutchinov
Jun 1, 2002·International Immunology·Simon J Prasad, Christopher C Goodnow
Sep 25, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Peter O Krutzik, Garry P Nolan
Jan 24, 2004·Clinical and Experimental Immunology·A C VasquezP A Morel
Jun 9, 2004·The Journal of Experimental Medicine·Kristin V TarbellRalph M Steinman
Mar 30, 2005·The Journal of Experimental Medicine·Carine Asselin-PaturelGiorgio Trinchieri
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Jan 6, 2007·Science·Diana DudziakMichel C Nussenzweig
Jan 11, 2007·The Journal of Experimental Medicine·Kristin V TarbellRalph M Steinman
Mar 3, 2010·Immunological Reviews·Ken Shortman, William R Heath
Mar 3, 2010·Immunological Reviews·Pilar M Domínguez, Carlos Ardavín
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Annie W Lau-KilbyKristin V Tarbell
Dec 6, 2011·Annual Review of Immunology·Ralph M Steinman
May 5, 2012·PloS One·Joshua N BeilkeLewis L Lanier
Jul 17, 2012·Nature Immunology·Jennifer C MillerUNKNOWN Immunological Genome Consortium
Aug 3, 2012·Cold Spring Harbor Perspectives in Biology·Kanako L Lewis, Boris Reizis
Dec 25, 2013·Frontiers in Immunology·Penelope A Morel
May 20, 2014·Immunity·Alexander Mildner, Steffen Jung
Jul 19, 2014·Nature Reviews. Immunology·Martin GuilliamsSimon Yona
Jun 14, 2015·Diabetes·Jeffrey D PriceKristin V Tarbell

❮ Previous
Next ❯

Citations

Apr 19, 2016·Journal of Immunological Methods·Kang Liu, Bernard Malissen
Dec 8, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joanne A O'DonnellMichelle A Kelliher
Jan 23, 2016·Journal of Leukocyte Biology·Chie Hotta-Iwamura, Kristin V Tarbell
May 28, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joanna E KlementowiczQizhi Tang
Jan 18, 2020·Science·Mohamed A ElTanboulyRandolph J Noelle
Aug 26, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·So Ri JungMarkus J Hofer
Jan 2, 2021·Frontiers in Immunology·Damilola Oyewole-SaidWilliam K Decker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.