PMID: 2107260Mar 9, 1990Paper

Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator

Journal of Immunological Methods
P A Vandenberghe, J L Ceuppens

Abstract

Cytoplasmic free calcium [( Ca2+]i) is a key intracellular messenger in many cell types. We have used fluo-3, a recently developed calcium probe, to study [Ca2+]i in resting and stimulated human peripheral blood T lymphocytes. The spectral properties of fluo-3 permit analysis of [Ca2+]i in flow cytometers with a 488 nm argon laser excitation source and fluorescein filter settings. The data obtained with fluo-3 are both qualitatively and quantitatively in good agreement with the data in the literature. After stimulation of T lymphocytes with the mitogens phytohaemagglutinin, concanavalin A and with OKT3, and anti-CD3 monoclonal antibody, a biphasic [Ca2+]i response was observed, with an early EGTA-insensitive [Ca2+]i rise, followed by an EGTA-sensitive sustained [Ca2+]i plateau. Non-mitogenic monoclonal antibodies directed against the CD5, CD28 and CD7 T cell surface antigens elicited [Ca2+]i rises only when crosslinked on the cell surface with goat anti-mouse IgG. Conversion of fluorescence data into absolute [Ca2+]i values by means of a non-disruptive calibration procedure, yielded a [Ca2+]i of 107 +/- 18 nM (mean +/- SD, n = 13) in resting T lymphocytes. Time-dependent loss of cellular dye content limits the precision of the ...Continue Reading

References

Apr 24, 1986·The New England Journal of Medicine·H Rasmussen
Jan 1, 1986·Annual Review of Immunology·A WeissJ Stobo
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J A LedbetterP S Rabinovitch
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Sep 1, 1986·Cellular Immunology·S J MentzerD V Faller
May 1, 1985·Analytical Biochemistry·C M DeberS Grinstein
Apr 1, 1985·Cell Calcium·T J Rink, T Pozzan
Dec 1, 1984·Journal of Cellular Physiology·E W GelfandS Grinstein

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Citations

Mar 15, 1996·Journal of Cellular Biochemistry·M OkuyamaM Monden
Jun 2, 2000·Biochemical and Biophysical Research Communications·M MaccarroneA Finazzi-Agrò
Sep 26, 2012·Biological Trace Element Research·Durga AttiliJames Varani
May 1, 1995·Human Immunology·A M VanBuskirkC G Orosz
Apr 1, 1992·Current Opinion in Immunology·C H June
Feb 1, 1993·Current Opinion in Biotechnology·G van den Engh
Mar 22, 2002·Developmental and Comparative Immunology·Osamu Kurata, Kishio Hatai
Apr 27, 1999·Immunology Letters·D Forcić, R Mazuran
Apr 23, 1999·Neuroscience Letters·A A BoldyrevD O Carpenter
Nov 21, 1998·Free Radical Biology & Medicine·V A DyatlovD O Carpenter
Oct 2, 2002·Journal of Neurochemistry·Mauro MaccarroneAlessandro Finazzi-Agrò
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S GreenbergB Seed
Nov 23, 2005·The Journal of Experimental Medicine·Philip SmithPadraic G Fallon
Jul 30, 1999·British Journal of Obstetrics and Gynaecology·P von DadelszenC W Redman
Apr 4, 2000·American Journal of Respiratory Cell and Molecular Biology·S I AhmedP J Woll
Mar 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hagen PommerenkeJoachim Rychly
Feb 24, 1998·Clinical and Experimental Immunology·G D Appleyard, B N Wilkie
Jan 15, 2014·Chemico-biological Interactions·Masanori HorieHitoshi Iwahashi
Nov 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bartosz ChmielowskiLeszek Ignatowicz
Nov 11, 2014·Oxidative Medicine and Cellular Longevity·Taichi KashiwagiSanetaka Shirahata
Jul 21, 2009·Journal of Immunological Methods·Eva SchepersRaymond Vanholder
Jul 19, 2008·Clinical Biochemistry·Laura BazzichiAntonio Lucacchini
May 6, 2004·Basic & Clinical Pharmacology & Toxicology·Irena ManovTheodore C Iancu
Mar 26, 2004·Bioelectromagnetics·Shinji HarakawaDavid E Martin
Jan 15, 2013·Journal of Biochemical and Molecular Toxicology·Neelima PathakShashi Khandelwal

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