PMID: 7018625Jun 1, 1981Paper

Flow-cytometric measurements of the transmembrane potential, the surface charge density and the phagocytic activity of Guinea pig macrophages after incubation with lymphokines

Blut
G ValetG Ruhenstroth-Bauer

Abstract

The effects of lymphokines on guinea pig peritoneal macrophages were measured via flow cytometry utilizing the three-parameter FLUVO-METRICELL flow cytometer. On the basis of cell volume three distinct macrophage populations could be distinguished. Three to 5 min after starting the incubation with lymphokines a hyperpolarization of all three macrophage populations took place which was followed by depolarization. After 60 min the transmembrane potential reached again its control values. The negative charge density of the cell membrane decreased shortly after beginning of the incubation to 70-80% of the initial value and then remained unchanged for the following 120 min. The phagocytic activity of the macrophages was diminished during the depolarisation phase but increased over control values after restoration of the transmembrane potential.

References

Feb 1, 1978·The Journal of Experimental Medicine·L H BlockG Ruhenstroth-Bauer
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G ValetG Ruhenstroth-Bauer
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H M ShapiroL A Kamentsky
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V KachelG Ruhenstroth-Bauer
Jan 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V Kachel

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Citations

Jan 1, 1991·Pflügers Archiv : European journal of physiology·A MarxR Rüdel
Sep 1, 1990·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·R J Foerster, G Wolf
Aug 23, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Attila Tárnok, Gero Brockhoff
Sep 15, 1982·Analytical Biochemistry·H S Kruth
Oct 13, 2017·European Journal of Immunology·Andrea CossarizzaJakob Zimmermann
Jan 1, 1987·Scandinavian Journal of Infectious Diseases·C DahlgrenS Eriksson
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky

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