PMID: 16511791Mar 3, 2006Paper

Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45

Veterinary Clinical Pathology
S ComazziPaola Sartorelli

Abstract

In dogs, flow cytometry is used in the phenotyping of immunologic cells and in the diagnosis of hemic neoplasia. However, the paucity of specific antibodies for myeloid cells and B lymphocytes and of labeled antibodies for multicolor techniques limits the ability to detect all leukocyte subpopulations. This is especially true for neoplastic and precursor cells. CD18 and CD45 are expressed on all leukocytes and are involved in cell activation, and together could be useful in helping determine cell lineage. The purpose of this study was to double label canine blood for CD18 and CD45 and to use the differential expression of antigens to identify leukocyte populations in dogs with non-neoplastic and neoplastic hematologic diseases. A template was developed using blood samples from 10 clinically healthy dogs and a back-gating technique. Differential leukocyte counts obtained with the template were compared with those obtained by manual and automated methods on blood samples from 17 additional healthy dogs. Blood samples obtained from 9 dogs with non-neoplastic (reactive) hematologic diseases and 27 dogs with hemic neoplasia were double stained for CD18 and CD45 using mouse anticanine CD18 monoclonal antibody (mAb) plus phycoerythrin...Continue Reading

Citations

Jan 28, 2009·Veterinary Clinical Pathology·Silvia RossiStefano Comazzi
Nov 26, 2010·Journal of Veterinary Internal Medicine·S ComazziM Mortarino
Mar 29, 2011·The Veterinary Journal·Fulvio Riondato
Feb 8, 2011·Clinics in Laboratory Medicine·Leslie C Sharkey, Maxey L Wellman
Apr 2, 2016·Veterinary Immunology and Immunopathology·F RiondatoB Miniscalco
Jun 1, 2013·Veterinary and Comparative Oncology·V MartiniS Comazzi
Sep 10, 2010·Veterinary Pathology·F Reggeti, D Bienzle
Jul 29, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Valeria MartiniStefano Comazzi
Sep 3, 2010·Journal of the American Animal Hospital Association·Stefano ComazziPaola Roccabianca
May 31, 2012·The Veterinary Record·C LocatelliS Comazzi
Jul 15, 2009·The Veterinary Record·G RossiS Comazzi
Apr 20, 2021·Frontiers in Veterinary Science·Fulvio Riondato, Stefano Comazzi

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