PMID: 9428182Jan 15, 1998Paper

Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans

Revista Da Sociedade Brasileira De Medicina Tropical
J M Egido, J Viñuelas

Abstract

We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripheral blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1 micron diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripheral blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.

References

Jul 5, 1991·Journal of Immunological Methods·D Gabrilovich, L Serebrovskaya
Aug 1, 1990·Pathology, Research and Practice·R B Ashman, J M Papadimitriou
May 1, 1990·Journal of Neuroscience Research·F L JordanW E Thomas
Nov 30, 1989·Journal of Immunological Methods·H Buschmann, M Winter
Jan 1, 1989·Reviews of Infectious Diseases·R BjerknesC O Solberg
Jun 1, 1969·Journal of Bacteriology·R I Lehrer, M J Cline

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Citations

Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Feb 2, 2010·FEMS Immunology and Medical Microbiology·Lucia PaulovicováNikolay E Nifantiev
Jul 5, 2016·Photodiagnosis and Photodynamic Therapy·Filipe D S GorzaNara C de Souza
Jun 30, 2016·Photodiagnosis and Photodynamic Therapy·Kevin F Dos SantosNara C de Souza

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