Flow cytometric technique for determination of prostasomal quantity, size and expression of CD10, CD13, CD26 and CD59 in human seminal plasma

International Journal of Andrology
Lena CarlssonAnders Larsson

Abstract

Summary Prostasomes are prostate-derived organelles in seminal plasma exhibiting pluripotent properties to facilitate the fertilization process. Seminal prostasome concentration, size distribution and expression of the prostasomal surface antigens CD10, CD13, CD26 and CD59 were examined by flow cytometry. The study group consisted of 79 men with involuntary infertility. Very strong correlations existed between the prostasome expressions of the different CD markers. Significant correlations between prostasome concentration and CD molecules were weak or lacking. Further, no or weak relationships were observed between the prostasomal CD markers and sperm morphology, seminal fructose, neutral alpha-glucosidase activity, zinc and tumour necrosis factor alpha concentrations. Flow cytometry is a practical way to study prostasomes in seminal fluid without prior separation. This is a new technique for evaluation of the role of prostasomes and their functions in male reproductive physiology.

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Citations

Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Geng ZhangMark I Greene
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Gholamreza TavoosidanaMasood Kamali-Moghaddam
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lang ChenFang Li
Sep 2, 2010·PloS One·Regina Berretta, Pablo Moscato
Oct 24, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Marian AalbertsWillem Stoorvogel
Sep 4, 2012·Biochimica Et Biophysica Acta·Marian AalbertsWillem Stoorvogel
Apr 13, 2010·Immunobiology·R TarazonaJ G Casado
Jul 8, 2008·Trends in Molecular Medicine·Paola Mina-Osorio
Jan 15, 2009·The Prostate·K Göran RonquistAnders Larsson
Jun 4, 2011·Molecular Reproduction and Development·Göran K RonquistAnneli Stavreus-Evers
Oct 30, 2010·International Journal of Andrology·H Pons-RejrajiG Grizard
Aug 6, 2013·Systems Biology in Reproductive Medicine·Göran K RonquistAnders Larsson
May 28, 2013·Biochimica Et Biophysica Acta·K Göran RonquistAnders Larsson
Apr 13, 2013·Journal of Reproductive Immunology·C FranzB Toth
Nov 2, 2014·The Journal of Biological Chemistry·Chang LiuFang Li

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