Flow cytometric techniques for the detection of microorganisms

Methods in Cell Science : an Official Journal of the Society for in Vitro Biology
Hazel M Davey

Abstract

Flow cytometry (FCM) is a technique, which allows one to analyse cells rapidly and individually, and permits the quantitative analysis of distributions of a property or properties in a population. It therefore offers many advantages over conventional measurements for the analysis of biological cells. Historically the technique has been widely applied for the study of mammalian cells, but its use in microbiology has been more limited; this is mainly a consequence of the smaller size of microbes, which results in the smaller optical signals that can be obtained from them. Developments in light sources and optics, together with brighter, spectrally-diverse dyes have reduced this barrier over recent years and the flow cytometer is now an essential tool in many microbiological research establishments. FCM has an increasing role to play in the detection of microbes in both industrial and clinical settings. Environmental monitoring to prevent outbreaks of human diseases such as cryptosporidiosis and Legionnaires' disease and to detect acts of biowarfare or bioterrorism are all amenable to flow cytometric study. This review seeks to highlight the role of the flow cytometer in the detection of microbial cells.

Citations

Mar 31, 2010·Journal of Fluorescence·Filomena SilvaFernanda Conceição Domingues
Aug 21, 2010·Immunology·Namrata Tomar, Rajat K De
Jan 4, 2019·Archives of Microbiology·Olga D HendricksonBoris B Dzantiev
Sep 26, 2020·Pharmaceutics·Jordan PascoeJoanna Bacon
Mar 21, 2009·Extremophiles : Life Under Extreme Conditions·Peter L BergquistTristrom Winsley
Jul 22, 2019·Archives of Toxicology·Charlotte EsserHyun-Dong Chang
Mar 10, 2006·Applied Microbiology and Biotechnology·Anfeng XiaoYuanxing Zhang
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker

Related Concepts

Environmental Microbiology
Flow Cytometry
Yeasts
Streak Plate Count

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