Flow cytometric validation of automated differentials in pediatric patients

Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology
Laura S FinnJoe C Rutledge

Abstract

Manual differential white blood cell (WBC) counting has been considered the gold standard and is routinely used to validate differentials obtained with other methodologies. To validate the accuracy of automated lymphocyte counts, we compared 2-part differentials using a Coulter HmX hematology analyzer and a Coulter XL flow cytometer to analyze 57 pediatric samples with WBC counts ranging from 0.7 k to 33.4 k. These data were compared with manual differential counts. We found excellent correlation between the two automated lymphocyte and monocyte counting methods that surpassed the manual correlations, indicating manual lymphocyte or monocyte counts are unnecessary in the setting of quality scatterplots. To evaluate the use of automated differentials for our most labor-intensive cases (low WBCs, which frequently require manual differentials) we then compared 3-part differentials using the HmX hematology analyzer and flow cytometer for 51 samples with total WBC < or = 1.1 x 10(9)/L. Manual differentials (< or = 100-cell counts) were available on 27 samples. Although the correlations for manual versus automated or flow differentials were good for all cell types, the correlation between the two automated methods was better, irrespe...Continue Reading

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Citations

Mar 18, 2009·American Journal of Clinical Pathology·Maria A Proytcheva
Sep 20, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jean-Luc FaucherJean Feuillard
Jun 10, 2010·Cytometry. Part B, Clinical Cytometry·Sindhu CherianBrent L Wood
Jun 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mikael RousselUNKNOWN International Council for Standardization in Hematology (ICSH)
May 28, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mikael RousselThierry Fest
Feb 14, 2015·Clinics in Laboratory Medicine·Edward C C Wong

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