PMID: 9539458Apr 16, 1998Paper

Flow cytometry analysis of gingival and periodontal ligament cells

Journal of Dental Research
L KuruI Olsen

Abstract

Gingival and periodontal ligament (PDL) fibroblasts are the major cellular components of periodontal soft connective tissues, but the precise differences between these cells are not yet known. In the present study, we have therefore examined the phenotypic and functional features of the cells obtained from gingival and PDL biopsy samples. Spindle-shaped cells characteristic of fibroblasts were the main cell type observed in vitro, although epithelial cells were also present in primary gingival cell cultures. Flow cytometry was used to measure the size and granularity of the cultured cells, and showed that the gingival fibroblasts were smaller and less granular compared with the PDL cells. The expression of certain key extracellular matrix (ECM) proteins, fibronectin, collagen type I, and tenascin was measured by flow cytometry. Analysis of the fluorescence profiles of these cultures showed that the majority of cells expressed fibronectin and that the average fluorescence intensity of this antigen in the PDL cells was higher than that in the gingival fibroblasts. Moreover, the fibronectin-positive PDL cells apparently comprised two subpopulations which expressed fibronectin at different levels, suggesting that the cells in the P...Continue Reading

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Citations

Apr 17, 2001·Biochemical and Biophysical Research Communications·J C ParkK Y Han
Apr 20, 1999·Journal of Periodontal Research·L KuruI Olsen
Sep 30, 2003·Journal of Periodontology·Xiaozhe Han, Salomon Amar
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May 29, 2004·Journal of Endodontics·Won-Gyun ChungJin Kim

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