PMID: 9647805Jul 2, 1998Paper

Flow cytometry detection of infectious rotaviruses in environmental and clinical samples

Applied and Environmental Microbiology
F X AbadA Bosch

Abstract

A method for the detection of infectious human rotaviruses based on infection of CaCo-2 cells and detection of infected cells by indirect immunofluorescence and flow cytometry (IIF-FC) has been developed. The technique was validated by performing a seminested reverse transcription-PCR assay with sorted cell populations. The efficiency of the procedure has been compared with that of the standard method of infection of MA104 cells and ulterior detection by IIF and optical microscopy (IIF-OM) and with that of infection of MA104 cells and detection by IIF-FC. The limit of sensitivity for the detection of the cell-adapted strain Ito(r) P13, expressed as the most probable number of cytopathogenic units, was established as 200 and 2 for MA104 and CaCo-2 cells, respectively, by the IIF-FC method. The ratio of infectious virus particles to total virus particles for a wild-type rotavirus was determined to be 1/2 x 10(6) and 1/2 x 10(4) for IIF-OM with MA104 cells and IIF-FC with CaCo-2 cells, respectively. The use of IIF-FC with CaCo-2 cells was tested with fecal and water samples and proved to be more effective than the standard procedure for rotavirus detection.

References

May 1, 1992·Journal of Virological Methods·W O GrabowA Bosch
May 1, 1991·Reviews of Infectious Diseases·S A AnsariS A Sattar
Jan 1, 1981·Archives of Virology·K SatoM Matumoto
Jul 1, 1983·Journal of Clinical Microbiology·C D BrandtR H Parrott
Sep 1, 1995·Applied and Environmental Microbiology·R GajardoA Bosch

❮ Previous
Next ❯

Citations

Feb 20, 2002·Journal of Applied Microbiology·A P Wyn-Jones, J Sellwood
Apr 10, 2009·Environmental Monitoring and Assessment·Tiziana GrassiAntonella De Donno
Jun 1, 2000·Applied and Environmental Microbiology·B Gratacap-CavallierJ M Seigneurin
Oct 17, 2006·Journal of Applied Microbiology·E MelegG Szucs
Dec 15, 2020·Journal of Hazardous Materials·Mohsen PilevarWoo Hyoung Lee
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Jul 6, 2010·Biomacromolecules·Seung-Hoon BaekJeong Hill Park
Sep 17, 2011·International Journal of Hygiene and Environmental Health·Ibrahim Ahmed HamzaMichael Wilhelm

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.