PMID: 9451557Feb 6, 1998Paper

Flow cytometry in diagnostic cytology

Diagnostic Cytopathology
T J O'Leary

Abstract

Flow cytometry (FCM) is a useful adjunct to cytologic examination, because the quantitative biochemical information it provides complements the morphologic information gained during visual examination. It aids in the interpretation of bladder washings, and is particularly useful for the assessment of lymphoid lesions, whether they originate from fine-needle aspiration, cerebrospinal fluid, or effusions. Optimal use of FCM frequently requires assessment of more than one parameter; simultaneous use of cell differentiation markers and nuclear DNA quantitation is often significantly more useful than either alone. Despite the utility of FCM, however, the potential for future development appears to be limited. Improvements in image cytometry allow reasonable assessment of ploidy and S-fraction to be made from specimens prepared on glass slides. Multiparameter measurements may also be accomplished with imaging techniques, which allow the further advantage of visual identification of cells with equivocal morphologic changes. The development of artificial intelligence methods for use with imaging technology has also significantly exceeded that of FCM. Finally, image cytometry is often more useful for samples with few cells. Other challe...Continue Reading

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Citations

Nov 13, 2003·Postgraduate Medical Journal·S K Mohanty, P Dey
Feb 21, 2014·Diagnostic Cytopathology·Gordon H YuRebecca L King
May 26, 2001·The American Journal of Gastroenterology·C T BothM D Reis
Mar 10, 2001·Diagnostic Cytopathology·R BraunschweigJ Benhattar
Oct 13, 2001·Diagnostic Cytopathology·R BraunschweigJ Benhattar
Feb 27, 2016·Biomedical Engineering and Computational Biology·Abraham PouliakisPetros Karakitsos
Nov 10, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Awtar KrishanMehrdad Nadji
Mar 29, 2002·Survey of Ophthalmology·Russell W ReadNarsing A Rao
Jan 2, 2009·Animal Reproduction Science·R E G RiciM A Miglino
Apr 25, 2019·Acta Cytologica·Gordon H YuKaren S Gustafson

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