Flow cytometry isolation and improved visualization of sorted mouse chromosomes. Purification of chromosomes X and ISO-1 from cell lines with Robertsonian translocations

Experimental Cell Research
B BaronM E Goldberg

Abstract

While analysis and sorting of human chromosomes by flow cytometry has been widely used, isolation of a pure mouse chromosome remains very difficult, since most murine chromosomes are quite similar in size. To overcome this problem, we have analysed mouse cell lines having either Robertsonian translocations or isochromosomes. The resulting metacentric chromosomes are very different in size and in morphology from normal mouse acrocentric chromosomes. These characteristics have been analysed by computer-monitored flow cytometry, facilitated by improvements in the chromosome extraction procedure. Signals characteristic of the iso-lq chromosome in cell line PCC4 azaR1, and of the normal X chromosome in the mouse strain 22CD have thus been obtained. These chromosomes have been sorted and can be easily recognized by fluorescence microscopy when collected onto serum-albumin-coated microscope slides. The technical modifications made, coupled with the existence of a great diversity of metacentric chromosomes resulting from Robertsonian translocations, should allow the purification of a number of different mouse chromosomes.

References

Apr 1, 1979·The Journal of Cell Biology·A B BlumenthalJ W Sedat
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R V LeboY W Kan
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J W GrayM L Mendelsohn
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B D YoungE Boyd
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R KrumlaufB D Young

❮ Previous
Next ❯

Citations

Sep 13, 2001·Molecular Reproduction and Development·S Santucci-DarmaninV Paquis-Flucklinger
Jun 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A SchmitzE P Cribiu
May 1, 1986·Somatic Cell and Molecular Genetics·B BaronC Bishop
Jul 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M FernandesG F Carle
Apr 8, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M FernandesG F Carle
Jan 1, 1993·Biology of the Cell·P MétézeauG Frelat
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Z WirschubskyJ Sümegi
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P AvnerL B Russell
May 1, 1986·Experimental Cell Research·D Hernandez-VerdunM Bouteille

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Mammalian Genome : Official Journal of the International Mammalian Genome Society
P WilliamsonY Boyd
Mammalian Genome : Official Journal of the International Mammalian Genome Society
M FernandesG F Carle
© 2021 Meta ULC. All rights reserved