PMID: 7819587Jan 1, 1994Paper

Flow cytometry: potential utility in monitoring drug effects in breast cancer

Breast Cancer Research and Treatment
S KoesterM W DeGregorio

Abstract

Flow cytometric analysis of DNA ploidy and S-phase fraction are well recognized prognostic indicators in breast cancer. The present paper deals with the widening of the applications of flow cytometry to monitoring the effectiveness of antiestrogen therapy, detecting clonal selection and emergence of drug resistance, and monitoring chemosensitizing properties of drugs. Antiestrogen activity can be studied by DNA flow cytometry to address clinical research problems such as patient-specific pharmacokinetics, dosing compliance, and acquired antiestrogen resistance. Patient plasma specimens containing various concentrations of triphenylethylenes can be monitored for drug-induced effects using cell cycle measurements and correlated to in vivo drug levels. DNA flow cytometry has also been instrumental in the study of the effects of prolonged low-dose (0.5 microM for > 100 days) tamoxifen treatment on human estrogen receptor negative MDA-MB-231 cells, where it was shown that tamoxifen may significantly alter cell cycle kinetics and tumorigenicity of these cells, selecting a new, more aggressive, and rapidly growing clone. Lastly, it has been shown that the chemosensitizing properties of another triphenylethylene antiestrogen, toremifen...Continue Reading

References

Jun 1, 1992·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·V J WiebeM W DeGregorio
Jan 16, 1991·Journal of the National Cancer Institute·P VerrelleJ Chassagne
May 1, 1991·British Journal of Cancer·K Nooter, H Herweijer
Sep 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M W DeGregorioV J Wiebe
Mar 9, 1989·The New England Journal of Medicine·G M ClarkW L McGuire
Aug 1, 1987·European Journal of Cancer & Clinical Oncology·C K OsborneJ P Robinson
Jan 1, 1984·Pharmacology & Therapeutics·B J Furr, V C Jordan
Jan 1, 1993·Breast Cancer Research and Treatment·K B Horwitz
Jan 1, 1993·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P E SipilaM W DeGregorio
Jun 1, 1993·Critical Reviews in Oncology/hematology·V J WiebeM W DeGregorio

Citations

Sep 15, 2000·Journal of Immunological Methods·S K Koester, W E Bolton
Jun 20, 2012·Journal of Biophotonics·Susan M Daly, Martin J Leahy

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