Flow dynamics control endothelial permeability in a microfluidic vessel bifurcation model

Lab on a Chip
Ehsan AkbariJonathan W Song

Abstract

Endothelial barrier function is known to be regulated by a number of molecular mechanisms; however, the role of biomechanical signals associated with blood flow is comparatively less explored. Biomimetic microfluidic models comprised of vessel analogues that are lined with endothelial cells (ECs) have been developed to help answer several fundamental questions in endothelial mechanobiology. However, previously described microfluidic models have been primarily restricted to single straight or two parallel vessel analogues, which do not model the bifurcating vessel networks typically present in physiology. Therefore, the effects of hemodynamic stresses that arise due to bifurcating vessel geometries on ECs are not well understood. Here, we introduce and characterize a microfluidic model that mimics both the flow conditions and the endothelial/extracellular matrix (ECM) architecture of bifurcating blood vessels to systematically monitor changes in endothelial permeability mediated by the local flow dynamics at specific locations along the bifurcating vessel structure. We show that bifurcated fluid flow (BFF) that arises only at the base of a vessel bifurcation and is characterized by stagnation pressure of ∼38 dyn cm-2 and approxi...Continue Reading

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Citations

Apr 5, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Chia-Wen ChangJonathan W Song
Feb 27, 2020·Advanced Healthcare Materials·Shantanu PradhanJohn H Slater
Jul 7, 2020·Frontiers in Physiology·Emma GordonMaike Frye
Jul 3, 2020·Lab on a Chip·Mary Kathryn Sewell-LoftinGregory D Longmore
Aug 9, 2019·Biomedical Engineering Online·Ruomeng ChenDichen Li
Nov 13, 2020·Frontiers in Physiology·Ricardo SantamaríaAlicia G Arroyo
May 20, 2021·Lab on a Chip·Elisa M WassonMonica L Moya
Mar 25, 2021·American Journal of Physiology. Cell Physiology·Shelby C InbodyRenita E Horton
Jun 26, 2021·ACS Biomaterials Science & Engineering·Christos Panagiotis TasiopoulosMy Hedhammar
Jul 14, 2021·Journal of Biomechanical Engineering·Meghan E FallonMonica T Hinds
Oct 1, 2021·Biofabrication·Claire A DessallesAbdul I Barakat
Nov 25, 2021·Soft Matter·Paul F SalipanteStella Alimperti

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Methods Mentioned

BETA
biopsy
chips
fluorescence imaging

Software Mentioned

AutoCAD
CadArt
MATLAB
AutoDesk
COMSOL Multiphysics

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