PMID: 8399147Sep 28, 1993

Flow-force relationships in lettuce thylakoids. 2. Effect of the uncoupler FCCP on local proton resistances at the ATPase level

C SigalatF Haraux


The relationship between the steady-state proton gradient (delta pH) and the rate of phosphorylation was investigated in thylakoids under various conditions. Under partial uncoupling by carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP), the rate of ATP synthesis was reduced by less than expected from the decrease of delta pH. This was observed in the case of the pyocyanine-mediated cyclic electron flow around photosystem 1, but not with the H2O-->photosystem 2-->cytochrome b6f-->photosystem 1-->methyl viologen system. In state 4, a unique relation was found between delta pH and the "phosphate potential", delta Gp, regardless of whether the energy level was controlled by light input or FCCP. The anomalous effect of FCCP on the rate of ATP synthesis disappeared when the ATPase was partially blocked by the reversible inhibitor venturicidin, but not in the presence of tentoxin, an irreversible inhibitor. These results are consistent with the existence of a small kinetic barrier for protons, limiting their access to the ATPase. This resistance would be collapsed by FCCP.


Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R J KesslerD E Green
Oct 11, 1978·Biochimica Et Biophysica Acta·J A SteeleR D Durbin
Feb 9, 1978·Biochimica Et Biophysica Acta·G F AzzoneS Massari
Sep 21, 1978·Biochimica Et Biophysica Acta·R J WILLIAMS
Jun 17, 1991·Biochimica Et Biophysica Acta·V PetronilliG F Azzone
May 24, 1990·Biochimica Et Biophysica Acta·I Ahmed, G Krishnamoorthy
Aug 11, 1986·FEBS Letters·H WoeldersK Van Dam
Jun 1, 1984·Journal of Bioenergetics and Biomembranes·R A Dilley, U Schreiber
Nov 15, 1981·The Biochemical Journal·H V WesterhoffK Van Dam
Jan 2, 1984·FEBS Letters·H V WesterhoffD B Kell
Jan 1, 1961·Journal of Theoretical Biology·R J WILLIAMS


Oct 16, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Abhishek BhattacharyaAnjan Kr Dasgupta

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
H(+)-Transporting ATP Synthase, Acyl-Phosphate-Linked
Hydrogen-Ion Concentration

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.