PMID: 8399147Sep 28, 1993

Flow-force relationships in lettuce thylakoids. 2. Effect of the uncoupler FCCP on local proton resistances at the ATPase level

Biochemistry
C SigalatF Haraux

Abstract

The relationship between the steady-state proton gradient (delta pH) and the rate of phosphorylation was investigated in thylakoids under various conditions. Under partial uncoupling by carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP), the rate of ATP synthesis was reduced by less than expected from the decrease of delta pH. This was observed in the case of the pyocyanine-mediated cyclic electron flow around photosystem 1, but not with the H2O-->photosystem 2-->cytochrome b6f-->photosystem 1-->methyl viologen system. In state 4, a unique relation was found between delta pH and the "phosphate potential", delta Gp, regardless of whether the energy level was controlled by light input or FCCP. The anomalous effect of FCCP on the rate of ATP synthesis disappeared when the ATPase was partially blocked by the reversible inhibitor venturicidin, but not in the presence of tentoxin, an irreversible inhibitor. These results are consistent with the existence of a small kinetic barrier for protons, limiting their access to the ATPase. This resistance would be collapsed by FCCP.

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R J KesslerD E Green
Oct 11, 1978·Biochimica Et Biophysica Acta·J A SteeleR D Durbin
Feb 9, 1978·Biochimica Et Biophysica Acta·G F AzzoneS Massari
Sep 21, 1978·Biochimica Et Biophysica Acta·R J WILLIAMS
Jun 17, 1991·Biochimica Et Biophysica Acta·V PetronilliG F Azzone
May 24, 1990·Biochimica Et Biophysica Acta·I Ahmed, G Krishnamoorthy
Aug 11, 1986·FEBS Letters·H WoeldersK Van Dam
Jun 1, 1984·Journal of Bioenergetics and Biomembranes·R A Dilley, U Schreiber
Nov 15, 1981·The Biochemical Journal·H V WesterhoffK Van Dam
Jan 2, 1984·FEBS Letters·H V WesterhoffD B Kell
Jan 1, 1961·Journal of Theoretical Biology·R J WILLIAMS

Citations

Oct 16, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Abhishek BhattacharyaAnjan Kr Dasgupta

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Etioplasts
H(+)-Transporting ATP Synthase, Acyl-Phosphate-Linked
Hydrogen-Ion Concentration
Pandavir
Valinomycin
Vegetables

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