PMID: 16524096Mar 10, 2006Paper

Flow gradient liquid chromatography using a coated anion exchange microcolumn

Journal of Separation Science
Ekaterina P NesterenkoBrett Paull

Abstract

Ion chromatography on a 4.0-mm-long (3.0 mm ID) ion exchange column is presented. Using a 10 mM phosphate buffer (pH 2.22) the separation of up to six UV-absorbing anions was obtained using the microcolumn, containing 5 microm RP support (Phenomenex Gemini) coated with the zwitterionic surfactant, (N-dodecyl-N,N-dimethylammonio) undecanoate. The short analytical column facilitated the application of a flow gradient programme over the flow range 0.3-5 mL/min resulting in optimum resolution of nitrite, nitrate, benzoate, iodide, thiocyanate and trichloroacetate in less than 10 min. The effect of both eluent concentration and pH on the retention of six selected anions was investigated, showing a strong pH capacity dependence. The microcolumn was found to exhibit no selectivity towards chloride and so was well suited to the analysis of saline samples. To illustrate this, the rapid analysis of a concentrated iodized table salt sample (20 g/L) was carried out. Following standard addition, a concentration of 3.55 +/- 0.05 microg iodide/g and 1.05 +/- 0.02 microg iodate/g in the solid salt sample was determined.

References

Sep 1, 1991·Irish Journal of Medical Science·D S RíordáinT Aherne
Nov 20, 2002·Journal of Chromatography. a·Eadaoin Twohill, Brett Paull
Jul 11, 2003·Journal of Pharmaceutical and Biomedical Analysis·Minh Nguyet A NguyenY Vander Heyden
Jan 25, 2005·The Analyst·Brett Paull, Pavel N Nesterenko
Mar 24, 2005·Journal of Chromatography. a·Igor MihelicTine Koloini
Mar 24, 2005·Journal of Chromatography. a·Galina A Platonova, Tatiana B Tennikova

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Citations

Feb 23, 2017·Journal of Separation Science·Jiří UrbanFrantisek Svec
Sep 30, 2009·Analytica Chimica Acta·Ekaterina P NesterenkoBrett Paull

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