Flow injection analysis system equipped with a newly designed electrochemiluminescent detector and its application for detection of 2-thiouracil

Analytical Chemistry
Yuwu ChiGuonan Chen

Abstract

A new flow injection analysis (FIA) system equipped with an electrochemiluminescent (ECL) detector has been developed and applied for the ECL detection of 2-thiouracil. The FIA-ECL system used a specially designed flow-through ECL thin-layer cell to reduce the dead volume, the IR drop across the cell, and the probability of accumulation of gas bubbles in the cell. It was thus envisioned to improve the detection limit of the FIA-ECL method. After being established, the new FIA-ECL system was used to investigate the ECL response of 2-thiouracil in the presence of the ECL of Ru(bpy)3(2+). It was found that 2-thiouracil could enhance the ECL of Ru(bpy)3(2+) over a wide pH range (pH 4.0-12.0). A highly sensitive method for detection of 2-thiouracil in biological samples was developed by the new FIA-ECL system after optimizing several experimental conditions, such as the applied potential of the working electrode, the pH value of the aqueous solution, the flow rate of carrier solution, and the concentration of Ru(bpy)3(2+).

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Citations

Sep 6, 2012·Bioanalysis·Joe Boison
Feb 14, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jianshe HuangTianyan You
Sep 8, 2010·Physical Chemistry Chemical Physics : PCCP·Longhua GuoGuonan Chen
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Jun 15, 2016·Analytical and Bioanalytical Chemistry·Marta M P S NevesPablo Fanjul-Bolado
Apr 26, 2008·Analytical Chemistry·Guifen JieJun-Jie Zhu

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